血清AngⅡ、Ang-(1-7)表达水平与多囊卵巢综合征患者卵巢间质血流的关系  

Relationship between Serum AngⅡand Ang-(1-7)Expression Levels and Ovarian Interstitial Blood Flow in Patients with Polycystic Ovary Syndrome

作　　者：宋洁[1] 吴隽姝 赵寅生 尹婷婷 杨卓[1] 魏玉业 SONG Jie;WU Juan-shu;ZHAO Yin-sheng;YIN Ting-ting;YANG Zhuo;WEI Yu-ye(Department of Clinical Laboratory,The 901 Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Hefei,Anhui,230031,China;Department of Gynaecology,The 901 Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Hefei,Anhui,230031,China)

机构地区：[1]中国人民解放军联勤保障部队第901医院检验科,安徽合肥230031 [2]中国人民解放军联勤保障部队第901医院妇科,安徽合肥230031

出　　处：《现代生物医学进展》2022年第20期3897-3901,共5页Progress in Modern Biomedicine

基　　金：安徽省自然科学基金项目(1708085QH204)。

摘　　要：目的:探究血清肾素-血管紧张素系统(RAS)相关因子[血管紧张素(Ang)Ⅱ、Ang-(1-7)]表达水平与多囊卵巢综合征(PCOS)患者卵巢间质血流的关系。方法:选取2020年7月至2021年10月于我院诊治的PCOS患者88例,按照胰岛素抵抗指数(HOMA-IR)将入选者分为两组,其中HOMA-IR≥2.69者分为胰岛素抵抗组(PCOS-IR组,n=42),HOMA-IR<2.69者分为非胰岛素抵抗组(PCOS-NIR组,n=46)。此外,选取同期于我院进行体检的健康女性志愿者90例为对照组。采用酶联免疫吸附法(ELISA)测定血清中的AngⅡ、Ang-(1-7)表达水平,同时采用EPIQ5型彩色多普勒超声仪对所有受试者在采血当天进行卵巢间质血流监测,并计算峰值血流速度(PSV)、搏动指数(PI)和阻力指数(RI)。采用Pearson检验分析PCOS患者AngⅡ、Ang-(1-7)与卵巢间质血流指标的相关性。应用多因素Logistic回归分析PCOS患者卵巢间质血流的影响因素。结果:相较于对照组,PCOS组AngⅡ、Ang-(1-7)、PSV、HOMA-IR水平明显更高,而PI、RI水平则明显更低(P<0.05);相较于PCOS-NIR组,PCOS-IR组AngⅡ、Ang-(1-7)、PSV、HOMA-IR水平明显更高,而PI、RI水平则明显更低(P<0.05);Pearson相关分析结果显示,AngⅡ、Ang-(1-7)与PSV、HOMA-IR均呈正相关(P<0.05),而与PI、RI均呈负相关(P<0.05);同时AngⅡ、Ang-(1-7)呈正相关(P<0.05)。经多因素Logistic回归分析显示,AngⅡ、Ang-(1-7)、HOMA-IR是PCOS患者卵巢间质血流的相关影响因素(P<0.05)。结论:AngⅡ、Ang-(1-7)在PCOS患者血清中呈高表达,二者之间呈正相关,且在伴有胰岛素抵抗的PCOS患者血清中表达水平进一步升高,两者表达失衡在PCOS患者卵巢间质血流异常增多中起重要作用。Objective:To explore the relationship between the serum renin angiotensin system related factors[angiotensin(Ang)Ⅱ,Ang-(1-7)]expression levels and ovarian interstitial blood flow in patients with polycystic ovary syndrome(PCOS).Methods:A total of 88 patients with PCOS who were diagnosed and treated in our hospital from July 2020 to October 2021 were selected,and the candidates were divided into two groups according to the insulin resistance index(HOMA-IR).Those with HOMA-IR≥2.69 were divided into insulin resistance group(PCOS-IR group,n=42),and those with HOMA-IR<2.69 were divided into non insulin resistance group(PCOS-NIR group,n=46).In addition,90 healthy female volunteers who underwent physical examination in our hospital were selected as the control group.The serum AngⅡand Ang-(1-7)expression levels were measured by enzyme-linked immunosorbent assay(ELISA).Meanwhile,the ovarian interstitial blood flow indexes of all subjects were monitored by EPIQ5 color Doppler ultrasound on the day of blood collection,and the peak blood flow velocity(PSV),pulsatility index(PI)and resistance index(RI)were calculated.Pearson test was used to study the correlation between AngⅡ,Ang-(1-7)and ovarian interstitial blood flow in patients with PCOS.Multivariate Logistic regression analysis was used to analyze the influencing factors of ovarian interstitial blood flow in patients with PCOS.Results:Compared with the control group,the levels of AngⅡ,Ang-(1-7),PSV and HOMA-IR in PCOS group were significantly higher,while the levels of PI and RI were significantly lower(P<0.05).Compared with PCOS-NIR group,the levels of AngⅡ,Ang-(1-7),PSV and HOMA-IR in PCOS-IR group were significantly higher,while the levels of PI and RI were significantly lower(P<0.05).Pearson correlation analysis showed that AngⅡand Ang-(1-7)were positively correlated with PSV and HOMA-IR(P<0.05),but negatively correlated with PI and RI(P<0.05).There was a positive correlation between AngⅡand Ang-(1-7)(P<0.05).Logistic multifactor regression analysis s

关 键 词：血管紧张素Ⅱ 血管紧张素-(1-7) 多囊卵巢综合征 卵巢间质血流 

分 类 号：R711.75[医药卫生—妇产科学]