“生物分离工程”综合性实验设计  

Design on Comprehensive Experiments of Biological Separation Engineering

作　　者：谢虹[1] 张彪[1] 陆盈盈 Xie Hong;Zhang Biao;Lu Yingying(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《广东化工》2022年第22期260-261,267,共3页Guangdong Chemical Industry

摘　　要：《生物分离工程》是生物工程专业的核心课程,具有综合性、工程性及实践性的特点。为了提高《生物分离工程》的教学质量,设计了“法夫酵母中虾青素的溶剂萃取”、“干辣椒中辣椒红素的硅胶柱色谱分离”和“甜叶菊中甜菊糖苷高效液相色谱含量测定”等3个综合性实验。实验内容涵盖溶剂萃取、浸取、絮凝、细胞破碎、固液分离、真空浓缩、色谱分离和高效液相色谱法等课程内容。3个综合性实验项目简便、易于操作,应用于多届生物工程专业的本科生的实验教学,激发了学生的学习兴趣,巩固了学生所学的理论知识,提高了学生的实践操作能力,取得了良好的教学效果。Biological Separation Engineering is a core course of bioengineering major,which is comprehensive,engineering and practical course.In order to improve the teaching quality of Biological Separation Engineering course,three comprehensive experiments including extraction of astaxanthin from Phaffia rhodozyma by solvent extraction,separation of capsanthin from dry pepper by silica column chromatography and determination of steviol glycosides by high performance liquid chromatography in Stevia rebaudiana Bertoni were designed.The experimental contents cover the course contents of solvent extraction,solid-liquid extraction,flocculation,cell-disruption,solid-liquid separation,vacuum concentration,chromatographic separation and high performance liquid chromatography method.The experiments are simple and easy to operate.The three experimental projects have stimulated undergraduates’learning interest,consolidated their theoretical knowledge,promoted their practical operation ability,and achieved good teaching results.

关 键 词：生物分离工程 综合性实验 设计 虾青素 辣椒红素 甜菊糖苷 

分 类 号：G642[文化科学—高等教育学] Q819[文化科学—教育学]