2-乙酰基毛蕊花糖苷抗破骨细胞形成及其作用机制  

2-Acetylacteoside Inhibits Osteoclast Formation and the Machanism

作　　者：韩晓玲 杨云 韦秋 蔡铭琪 毛浩萍[1] HAN Xiao-ling;YANG Yun;WEI Qiu;CAI Ming-qi;MAO Hao-ping(Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae,Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学方剂学教育部重点实验室,天津301617

出　　处：《中国现代中药》2022年第11期2150-2156,共7页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82174180,81630106)。

摘　　要：目的:研究2-乙酰基毛蕊花糖苷对破骨细胞分化的影响及潜在的分子机制。方法:采用抗酒石酸酸性磷酸酶(TRAP)染色及TRAP酶活力检测法评价0.1、1.0、10.0μmol·L^(-1)的2-乙酰基毛蕊花糖苷对破骨前体细胞向破骨细胞分化的影响;采用细胞增殖活性检测试剂盒(CCK8)法检测2-乙酰基毛蕊花糖苷对破骨细胞分化的影响;采用F-actin环染色和骨陷窝形成实验检测2-乙酰基毛蕊花糖苷对破骨前体细胞诱导形成的破骨细胞功能的影响;采用蛋白免疫印迹法(WB)检测破骨细胞分化相关特异性蛋白TRAP、整合素β3(ITGβ3)和细胞原癌基因c-Fos的表达水平。结果:与模型组相比,2-乙酰基毛蕊花糖苷显著降低TRAP活性(P<0.05),且对破骨前体细胞活力未见显著影响,提示2-乙酰基毛蕊花糖苷显著抑制破骨细胞形成。F-actin环染色和骨陷窝形成实验也显示2-乙酰基毛蕊花糖苷显著抑制破骨细胞骨吸收功能;WB实验结果表明2-乙酰基毛蕊花糖苷可显著抑制破骨细胞分化特异性蛋白TRAP、ITGβ3和c-Fos等蛋白水平的表达。结论:2-乙酰基毛蕊花糖苷能抑制破骨细胞的形成,作用机制可能与其抑制TRAP、ITGβ3和c-Fos的表达有关。Objective:To investigate the effect of 2-acetylacteoside on osteoclast differentiation and the underlying molecular mechanism.Methods:Firstly,the effects of 0.1,1.0,and 10.0μmol·L^(-1)2-acetylacteoside on the differentiation of osteoclast precursor cells into osteoclasts were evaluated by staining with tartrate-resistant acid phosphatase(TRAP)and detection of TRAP activity.Subsequently,Cell Counting Kit-8(CCK8)was used to examine the effect of 2-acetylacteoside on osteoclast differentiation.F-actin ring staining and bone lacuna formation assay were employed to detect the effect of 2-acetylacteoside on the function of osteoclasts derived from osteoclast precursor cells.Finally,Western blotting was employed to determine the expression of osteoclast differentiation-related specific proteins TRAP,integrin beta 3(ITGβ3),and c-Fos.Results:Compared with the model group,2-acetylacteoside reduced TRAP activity(P<0.05)and had no significant effect on the viability of osteoclast precursor cells,which suggested that 2-acetylacteoside significantly inhibited the formation of osteoclasts.Similarly,F-actin ring staining and bone lacuna formation experiments showed that 2-acetylacteoside significantly inhibits osteoclast-mediated bone resorption.Western blot showed that 2-acetylacteoside significantly down-regulated the expression of osteoclast differentiation-associated proteins such as TRAP,ITGβ3,and c-Fos.Conclusion:2-Acetylacteoside may inhibit the formation and function of osteoclasts by down-regulating the expression of TRAP,ITGβ3,and c-Fos.

关 键 词：破骨细胞 2-乙酰基毛蕊花糖苷 抗酒石酸酸性磷酸酶 整合素Β3 原癌基因C-FOS 

分 类 号：R285[医药卫生—中药学]