双醛纤维素纳米晶Pickering乳液的制备及其释放二氢杨梅素的研究  

Development and release behavior of dihydromyricetin-loaded Pickering emulsions stabilized by dialdehyde cellulose nanocrystals

作　　者：葛黎明[1] 徐锦芳 穆畅道[1] 李德富[1] GE Liming;XU Jinfang;MU Changdao;LI Defu(School of Chemical Engineering,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学化学工程学院,四川成都610065

出　　处：《食品与发酵工业》2022年第23期156-164,I0003-I0005,共12页Food and Fermentation Industries

基　　金：四川省应用基础研究项目(2021YJ0478);中央高校基本科研业务费专项资金项目(2021SCU12085)。

摘　　要：使用硫酸水解α-纤维素以制备得到纤维素纳米晶(cellulose nanocrystalline,CNC),然后利用高碘酸钠将CNC分子中邻位羟基特异性地氧化成醛基制得双醛纤维素纳米晶(dialdehyde cellulose nanocrystalline,DCNC)。分别以CNC和不同醛基含量的DCNC的悬浮液为水相,大豆油为油相制备负载二氢杨梅素的Pickering乳液。结果表明,通过调节高碘酸钠的添加量可以制备醛基含量可控的DCNC。当NaCl的浓度为50 mmol/L、CNC和DCNC悬液的质量浓度为10.0 g/L、油水相比为0.4∶1,采用超声-均质结合法能够制备稳定的Pickering乳液。高碘酸钠氧化能够减小CNC的粒径以及其表面的负电荷,进而显著增强Pickering乳液的稳定性。Pickering乳液对二氢杨梅素的释放速率随着DCNC醛基含量的增加而逐渐减缓。Pickering乳液缓释二氢杨梅素的动力学过程符合基于溶出和扩散机制的Weibull分布模型。综上,通过控制高碘酸钠对CNC的氧化程度,可以有效调节Pickering乳液的稳定性和缓释行为。Cellulose nanocrystalline(CNC)was prepared by the sulfuric acid hydrolysis ofα-cellulose.Then the ortho-hydroxyl group of CNC was specifically oxidized to the aldehyde group by sodium periodate to obtain dialdehyde cellulose nanocrystalline(DCNC).Dihydromyricetin-loaded Pickering emulsions were prepared using CNC and DCNC suspension as the aqueous phase and soybean oil as the oil phase.The results indicated that DCNC with controllable aldehyde group content could be prepared by adjusting the dosage of sodium periodate.Pickering emulsion prepared by the optimal parameters(10.0 g/L of DCNC3,0.4 of the oil/water ratio,50 mmol/L of salt ions)emulsified by the combination process of ultrasonic and homogeneous were very stable during storage and centrifugation.The reduced particle size and the surface negative charge of CNC by sodium periodate oxidation made a great contribution to significantly enhancing the stability of Pickering emulsion.The release rate of dihydromyricetin from DCNC stabilized Pickering emulsion was gradually slowed down with the increasing aldehyde content of DCNC.It was found that the sustained release of dihydromyricetin from Pickering emulsion conforms to the Weibull distribution model.The in vitro dihydromyricetin release mechanism from Pickering emulsion was based on dissolution and diffusion.Overall,the stability and sustained-release behavior of DCNC stabilized Pickering emulsion can be effectively adjusted by controlling the oxidation degree of CNC.

关 键 词：Pickering乳液 纤维素纳米晶 高碘酸钠氧化 二氢杨梅素 缓释 

分 类 号：O648.23[理学—物理化学] O629[理学—化学]