右美托咪定介导miR-526b-3p/MMP2通路影响人宫颈癌细胞株HeLa侵袭、迁移  

Dexmedetomidine mediated miR-526b-3p/MMP2 pathway affects the invasion and migration of human cervical cancer cell line HeLa

作　　者：张素冰[1] 赵滨滨[1] 高辉 张丹琦[1] ZHANG Subing;ZHAO Binbin;GAO Hui;ZHANG Danqi(Department of Anesthesia and Surgery,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院麻醉手术科,黑龙江哈尔滨150040

出　　处：《中国优生与遗传杂志》2022年第10期1703-1708,共6页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省中医药科研项目(ZHY2020-129)。

摘　　要：目的 探讨右美托咪定(Dex)介导微小核糖核酸-526b-3p(miR-526b-3p)/基质金属蛋白酶2(MMP2)通路影响人宫颈癌细胞株HeLa侵袭、迁移的作用。方法 采用不同浓度(0、10、20、40μmol/L)的Dex处理人宫颈癌细胞株HeLa并记为空白组、Dex低、中、高剂量组。Transwell小室实验检测细胞侵袭、迁移能力;实时定量聚合酶链反应(RT-qPCR)检测miR-526b-3p表达及MMP2信使核糖核酸(mRNA)表达。利用脂质体转染法分别将miR-526b-3p抑制物(anti-miR-526b-3p)及阴性对照(anti-miR-NC)、MMP2过表达载体(pcDNA-MMP2)及阴性对照(pcDNA-NC)转染至人宫颈癌细胞株HeLa,Transwell小室实验检测细胞侵袭、迁移能力;蛋白质免疫印迹法(WB)检测MMP2、MMP9、迁移侵袭增强因子1(MIEN1)蛋白表达;双荧光素酶报告实验验证miR-526b-3p靶向调控MMP2。结果 与空白组比,Dex低、中、高剂量组人宫颈癌细胞株HeLa侵袭和迁移细胞数目减少;与Dex+anti-miR-NC组比,Dex+anti-miR-526b-3p组侵袭和迁移细胞数目增加;与Dex+pcDNA-NC组比,Dex+pcDNA-MMP2组侵袭和迁移细胞数目增加(P<0.05)。与空白组比,Dex处理组miR-526b-3p表达升高,MMP2mRNA、MMP2、MMP9、MIEN1蛋白表达降低(P<0.05);与Dex+anti-miR-NC组比,Dex+anti-miR-526b-3p组MMP2、MMP9、MIEN1蛋白表达升高(P<0.05);与Dex+pcDNA-NC组比,Dex+pcDNA-MMP2组MMP2、MMP9、MIEN1蛋白表达升高(P<0.05)。结论 Dex可通过介导miR-219a-5p/MMP2通路降低人宫颈癌细胞株HeLa的侵袭、迁移能力。Objective To investigate the effect of dexmedetomidine(Dex) mediated micro ribonucleic acid-526b-3p(miR-526b-3p)/matrix metalloproteinase 2(MMP2) pathway on the invasion and migration of human cervical cancer cell line HeLa. Methods The human cervical cancer cell line HeLa was treated with Dex at different concentrations(0, 10, 20,40 μmol/L) and recorded as blank group, Dex low, medium and high dose groups. Transwell chamber assay was used to detect cell invasion and migration ability. Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of miR-526b-3p and MMP2 messenger ribonucleic acid(mRNA). The miR-526b-3p inhibitor(anti-miR-526b-3p) and negative control(anti-miR-NC), MMP2 overexpression vector(pcDNA-MMP2) and negative control(pcDNA-NC) were transfected into human cervical cancer cell line HeLa, and the cell invasion and migration ability were detected by Transwell assay. Western blotting(WB) was used to detect MMP2, MMP9, migration and invasion enhancer factor 1(MIEN1) protein expressions. Dual luciferase reporter assays verified that miR-526b-3p targets MMP2. Results Compared with the blank group,the number of invasive and migrating cells of human cervical cancer cell line HeLa decreased in the Dex low, medium and high dose groups, and compared with the Dex+anti-miR-NC group, the number of invasive and migrating cells in the Dex+anti-miR-526b-3p group increased, and compared with the Dex+pcDNA-NC group, the number of invasive and migrating cells in the Dex+pcDNA-MMP2 group increased(P<0.05). Compared with the blank group, the expression of miR-526b-3p in the Dex treatment group increased, and MMP2 mRNA expression, the protein expressions of MMP2, MMP9 and MIEN1 decreased(P<0.05). Compared with Dex+anti-miR-NC group, the protein expressions of MMP2, MMP9 and MIEN1 in Dex+anti-miR-526b-3p group increased(P<0.05). compared with Dex+pcDNA-NC group, the protein expressions of MMP2,MMP9 and MIEN1 in the Dex+pcDNA-MMP2 group increased(P<0.05). Conclusion Dex can reduce the i

关 键 词：右美托咪定 2宫颈癌 侵袭 迁移 

分 类 号：R614[医药卫生—麻醉学]