非变性质谱在天然产物研究中的应用  

Application of native mass spectrometry in natural product research

作　　者：朱达夫 唐春萍 刘佳 叶阳 ZHU Da-fu;TANG Chun-ping;LIU Jia;YE Yang(School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China)

机构地区：[1]上海科技大学生命科学与技术学院,上海201210 [2]中国科学院上海药物研究所,上海201203

出　　处：《药学学报》2022年第11期3292-3300,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81803610,21920102003);上海市科学技术委员会资助项目(20430780300);广东省重点领域研发计划项目(2020B0303070002)。

摘　　要：天然产物来源丰富、结构多样,是新药研发的重要源泉。从复杂的天然产物基质中高效筛选出有生物活性的成分是天然产物研究的重要方向之一。传统的研究方法从提取分离开始,然后进行结构鉴定和活性评价,耗时长、工作量大且带有很大的随机性,因此亟需发展高效的生物活性成分发现方法。非变性质谱(native MS)是一种基于靶标和配体相互作用的研究方法,能够根据两者之间的亲和力快速发现复杂基质中潜在的活性成分,具有特异、高效、简便的特点。本文简单介绍了非变性质谱的基本原理和影响因素,并综述了该方法近年来在天然产物研究领域中的应用,讨论了该方法的优势与局限性,以期为相关领域的研究者提供借鉴和参考。Natural products,rich in sources and diverse in structure,play an important role in drug discovery.It is a hotspot to identify efficiently bioactive components from complex mixtures in the field of natural product research.Traditional research methods,starting from extraction and isolation to structural elucidation,and finally to bioactivity assay,are generally time-consuming,laborious,and untargeted.Therefore,there is an urgent need to develop efficient screening methods for bioactive component discovery.Native mass spectrometry(native MS)is an underutilized method based on the interaction between target and ligand,which can rapidly discover potential active components in complex matrices.Due to the simple operation and the native state of the protein,the method is specific,efficient,and simple.Herein,we give a brief introduction of native MS and then review its recent application in natural product research,with its advantages and limitations,which would provide a reference for researchers in relevant fields.

关 键 词：非变性质谱 天然产物 生物亲和质谱 高通量筛选 分子间相互作用 

分 类 号：R917[医药卫生—药物分析学]