海星共附生真菌Aspergillus sp.WXF1904中一个新的异香豆素  

A new isocoumarin from a starfish-associated fungus Aspergillus sp.WXF1904

作　　者：汪玲玲[1] 管达 王晓芳[4] 庞小艳[2] 刘永宏[2,3] 林秀萍 WANG Ling-ling;GUAN Da;WANG Xiao-fang;PANG Xiao-yan;LIU Yong-hong;LIN Xiu-ping(Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,College of Life Sciences,South China Agricultural University,Guangzhou 510642,China;CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;Marine Environmental Engineering Center,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China)

机构地区：[1]华南农业大学生命科学学院,广东省农业生物蛋白质功能与调控重点实验室,广东广州510642 [2]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东广州510301 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458 [4]中国科学院南海海洋研究所,海洋环境工程中心,广东广州510301

出　　处：《药学学报》2022年第11期3356-3360,共5页Acta Pharmaceutica Sinica

基　　金：广东省自然科学基金资助项目(2020A1515010997);广东省海洋经济发展(海洋六大产业)专项资金项目(粤自然资合[2020]039)。

摘　　要：从南海一种海星样品中分离获得共附生真菌菌株WXF1904。经ITS区序列分析,鉴定该菌株属于曲霉属,命名为Aspergillus sp.WXF1904。采用正相硅胶柱色谱、ODS柱色谱和高效液相色谱等方法从Aspergillus sp.WXF1904发酵液粗浸膏中分离出7个化合物,根据各种波谱数据及结合文献分析,将其中1个鉴定为新的含卤素的异香豆素6-chloro-5,7-dihydroxy-3,8-dimethylisocoumarin(1),6个鉴定为已知化合物,分别为pilobolusate(2)、对羟基苯甲醛(3)、methyl orsellinate(4)、catechol(5)、vanillic acid(6)和wasabidienone E(7)。生物活性检测结果表明,化合物1和2具有微弱的乙酰胆碱酯酶抑制活性。A fungal strain WXF1904,was isolated from a starfish sample corrected in the South China Sea.According to its internal transcribed spacer(ITS)analysis,the strain was identified as a member of the genus Aspergillus and designated as Aspergillus sp.WXF1904.One new isocoumarin containing halogennamed 6-chloro-5,7-dihydroxy-3,8-dimethylisocoumarin(1),along with six known compounds pilobolusate(2),p-hydroxybenzaldehyde(3),methyl orsellinate(4),catechol(5),vanillic acid(6),and wasabidienone E(7),were isolated from the cultures of Aspergillus sp.WXF1904 by silica gel column chromatography,ODS gel column chromatography,and high performance liquid chromatography(HPLC).Their structures were elucidated by high resolution mass spectrometry(HR-MS),nuclear magnetic resonance(NMR)as well as literature comparison.The new compound 5-chloro-6,7-dihydroxy-3,8-dimethylisocoumarin and compound 2 showed weak acetylcholinesterase inhibitory activity.

关 键 词：海星共附生真菌 Aspergillus sp.WXF1904 次级代谢产物 异香豆素 乙酰胆碱酯酶抑制活性 

分 类 号：R284[医药卫生—中药学]