艾灸“肺肠同治”对哮喘模型大鼠肺功能及气道炎性反应的影响  

Effect of“joint treatment of lung and intestine”with moxibustion on lung function and airway inflammation in asthmatic rats

作　　者：周竞颖 来奕恬 丁攀婷 刘密[2] 李南[2] 张国山[2] 邱冉冉[3] ZHOU Jing-ying;LAI Yi-tian;DING Pan-ting;LIU Mi;LI Nan;ZHANG Guo-shan;QIU Ran-ran(Graduate School of Hunan University of Chinese Medicine,Changsha 410208,China;College of Acupuncture-moxibustion-Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208;The First Hospital of Hunan University of Chinese Medicine,Changsha410007)

机构地区：[1]湖南中医药大学研究生院,长沙410208 [2]湖南中医药大学针灸推拿与康复学院,长沙410208 [3]湖南中医药大学第一附属医院,长沙410007

出　　处：《针刺研究》2022年第11期969-974,共6页Acupuncture Research

基　　金：湖南省自然科学基金项目(No.2021JJ30513);湖南省教育厅科学研究项目(No.20B444);国家自然科学基金项目(No.81603705);中国博士后科学基金项目(No.2107M612567)。

摘　　要：目的:观察艾灸“天枢”“肺俞”对哮喘大鼠肺功能及气道炎性反应的影响,探讨“肺肠同治”在艾灸改善哮喘中的意义。方法:SD大鼠随机分为正常组、模型组、从肺论治组和肺肠同治组,每组12只。采用卵清白蛋白致敏和雾化激发法制备哮喘模型。从肺论治组大鼠艾灸双侧“肺俞”30 min;肺肠同治组大鼠艾灸双侧“肺俞”“天枢”30 min;模型组大鼠仅作固定30 min。上述3组大鼠干预结束1 h后,分别予以1%卵清白蛋白溶液雾化20 min,均每日1次,连续14 d。使用肺功能测定仪测定各组大鼠的肺功能;HE和Masson染色法观察各组大鼠肺组织病理形态学变化及胶原沉积程度;酶联免疫吸附法检测各组大鼠肺组织中白细胞介素(IL)-17、IL-4、IL-13、IL-33、IL-5、半胱氨酸白三烯(LT)、胸腺基质淋巴细胞生成素(TSLP)的含量。结果:与正常组比较,模型组大鼠肺功能中呼出25%肺活量时最大呼气流量(FEF 25%)、最大呼气中期流量(MMEF)、动态肺顺应性(Cdyn)、用力呼气量占用力肺活量比(FEV/FVC)、最大呼气流量(PEF)显著降低(P<0.05,P<0.01),肺阻力(RL)显著升高(P<0.05);肺组织结构重度异常,肺泡壁增厚,支气管周围可见大量炎性细胞浸润,肺组织胶原沉积程度显著升高(P<0.01);肺组织中IL-17、IL-4、IL-13、IL-33、IL-5、LT、TSLP含量均显著升高(P<0.01)。与模型组比较,从肺论治组大鼠肺功能中仅MMEF、Cdyn显著升高(P<0.05),肺肠同治组大鼠肺功能中FEF 25%、MMEF、Cdyn、FEV/FVC、PEF、RL均显著改善(P<0.01,P<0.05);从肺论治组和肺肠同治组大鼠肺泡壁增厚均较轻,支气管周围炎性细胞浸润明显减少,肺组织胶原沉积程度均显著降低(P<0.01);从肺论治组和肺肠同治组大鼠肺组织中IL-17、IL-4、TSLP含量均显著降低(P<0.05,P<0.01);肺肠同治组大鼠肺组织中IL-13、IL-33、IL-5、LT含量显著降低(P<0.05,P<0.01)。从肺论治组和肺肠同治组相比,肺组织中Objective To investigate the role of moxibustion of“Feishu”(BL13),“Tianshu”(ST25)for asthma by simultaneously treating lung and intestine(i.e.,treating both lung and intestine at the same time)in asthmatic rats.Methods A total of 48 SD rats were randomly divided into normal,model,lung treatment and joint-treatment of lung and intestine(joint-treatment)groups,with 12 rats in each.The asthma model was established by subcutaneous(bilateral back and inguinal regions)and intraperitoneal injection of mixture solution of albumin and Aluminium Hydroxide gel(on day 1^(st),and 9^(th))and followed by inhalation of atomized 1%ovalbumin(on day 15^(th),20 min each time,once daily for 1 week).Moxibustion was applied to bilateral BL13 for rats of the lung treatment group or bilateral BL13 and ST25 for rats of the joint-treatment group.One hour after the intervention,the rats in the later three groups were separately given nebulized 1%ovalbumin solution inhalation for 20 min.The treatments were conducted once daily for 14 consecutive days.After intervention,the lung functions including the forced expiratory flow 25%(FEF 25%),maximal mid-expiratory flow(MMEF),dynamic lung compliance(Cdyn),forced expiratory volume/forced vital capacity(FEV/FVC),peak expiratory flow(PEF),and lung resistance(RL)were measured by using a small animal lung function detector,and pathological changes and collagen deposition in the lung tissues were observed by H.E.and Masson staining,separately.The levels of interleukin(IL)-17,IL-4,IL-13,IL-33,IL-5,leukotriene(LT)and thymic stromal lymphocyte(TSLP)in the lung tissue were measured by ELISA.Results Compared with the normal group,the FEF 25%,MMEF,Cdyn,FEV/FVC and PEF were significantly decreased(P<0.05,P<0.01),and the pulmonary RL,collagen deposition,and contents of IL-17,IL-4,IL-13,IL-33,IL-5,TSLP and LT were notably increased(P<0.05,P<0.01)in the model group.After intervention,the MMEF and Cdyn in the lung treatment group,PEF,MMEF,Cdyn,FEV/FVC,FEF 25%in the joint-treatment group,were markedly incre

关 键 词：哮喘 艾灸 肺肠同治 气道炎性反应 肺功能 

分 类 号：R245.81[医药卫生—针灸推拿学]