吖啶橙-金纳米荧光法测金属硫蛋白  

Acridine orange-gold nanoparticles fluorescence method for detection of metallothioneins

作　　者：陈思涵 周鹏[2] 陈云生[2] 王永生[2] 王汉青[1,3] CHEN Si-han;ZHOU Peng;CHEN Yun-sheng;WANG Yong-sheng;WANG Han-qing(School of Resource Environment and Safety Engineering,University of South China,Hengyang 421001,China;School of Public Health,University of South China,Hengyang 421001,China;National and Local Joint Engineering Research Center for Airborne Pollutants Control and Radioactivity Protection in Buildings,Hengyang 421001,China)

机构地区：[1]南华大学资源环境与安全工程学院,湖南衡阳421001 [2]南华大学公共卫生学院,湖南衡阳421001 [3]建筑环境气载污染物治理与放射性防护国家地方联合工程研究中心,湖南衡阳421001

出　　处：《应用化工》2022年第11期3419-3423,共5页Applied Chemical Industry

基　　金：湖南省自然科学基金项目(2021JJ40470);湖南省教育厅优秀青年项目(19B491)。

摘　　要：吖啶橙荧光材料与柠檬酸根修饰的金纳米离子相互作用,通过表面能量转移,致荧光猝灭。在酸性条件下,金属硫蛋白与金纳米有更强结合能力,可以使体系荧光恢复。以λ_(ex)=493 nm为激发波长,测量加入金属硫蛋白前后λ_(em)=526 nm处的荧光变化值,线性范围为9.3×10^(-9)~1.5×10^(-7)mol/L,线性回归方程为ΔF=3.798+337.3C(×10^(-7)mol/L),r=0.9965,检测限为2.79×10^(-9)mol/L,相对标准偏差为0.39%~1.29%,加标回收率在97.09%~99.63%。实验方法已应用于人体尿液样品测定,具有选择性好、精密度和准确度高等优点。The interaction between acridine orange fluorescent dye and gold nanoparticles decorated by citrate leads to fluorescence surface energy transfer,resulting in fluorescence quenching.In the acid medium,metallothionein has stronger binding ability with gold nanoparticles,which can restore the fluorescence of the system.The change value of the fluorescence at λ_(ex)=526 nm is directly proportional to the concentracention of metallothionein in range of 9.3×10^(-9)~1.5×10^(-7)mol/L with the excitation wavelength of 493 nm.The regression equation of this method wasΔF=3.798+337.3C(×10^(-7)mol/L)with a correlation coefficient of 0.9965(r).The detection limit was 2.79×10^(-9)mol/L.The relative standard deviation were 0.39%~1.29%,recovery rate is between 97.09%and 99.63%.The method was used to detect the human urine samples with some advantages of better selectivity and higher precision and accuracy.

关 键 词：金属硫蛋白 金纳米粒子 吖啶橙 表面能量转移 分子荧光法 

分 类 号：TQ034[化学工程] R115[医药卫生—公共卫生与预防医学]