昆虫单个感觉器记录技术研究进展  

Research progress on insect single sensillum recording

作　　者：唐加菜 魏成梅 赵婧[1] 严乃胜[1] 董文霞[1] TANG Jia-cai;WEI Cheng-mei;ZHAO Jing;YAN Nai-sheng;DONG Wen-xia(College of Plant Protection,Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院/云南生物资源保护与利用国家重点实验室,昆明650201

出　　处：《应用生态学报》2022年第11期3146-3158,共13页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(31760636)资助。

摘　　要：单感器记录技术是一种昆虫细胞外电生理技术,可以测量昆虫单个感受器对刺激物的电生理反应。该技术有助于探明昆虫嗅觉和味觉感受器对不同信息化合物的电生理响应机制,将单感器记录技术与其他技术相结合,不仅可以阐明昆虫嗅觉反应的分子机制,还可以研制昆虫行为调节剂、检测挥发性有机化合物的生物传感器。本文介绍了单感器记录仪的结构和昆虫单感器记录的原理,并对单感器记录技术在昆虫学研究方面的应用进行了综述,以期为探明昆虫感受化学信息物质的机理和应用提供依据。Single sensillum recording is an insect extracellular electrophysiological technique,which can measure the electrophysiological responses of a single sensillum to stimuli in insects.It can help explore the electrophysiologi-cal response mechanism of insect olfactory and taste receptors to different semiochemicals.Combined with other techniques,it can be used to understand the molecular genetic mechanisms of olfactory responses,as well as to develop behavior regulators and volatile organic compound biosensors.In this review,we proposed the basic structure and the principle of single sensillum recording,and summarized its applications in insect studies,aiming to provide the foundation for understanding the mechanisms and applications in insect perception of semiochemicals.

关 键 词：单感器记录 昆虫 嗅觉感器 味觉感器 电生理反应 

分 类 号：Q965[生物学—昆虫学]