SRBSDV对抗白背飞虱水稻抗虫性的影响  

Effects of SRBSDV on the resistance of WBPH-resistant rice varieties

作　　者：黄所生[1,2,3] 秦碧霞 吴碧球[1,2,3] 谢慧婷 李成[1,2,3] 李战彪 黄凤宽[1,2,3] 蔡健和 HUANG Suo-Sheng;QIN Bi-Xia;WU Bi-Qiu;XIE Hui-Ting;LI Cheng;LI Zhan-Biao;HUANG Feng-Kuan;CAI Jian-He(Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs,Nanning 530007,China;Guangxi Key Laboratory for Biology of Crop Diseases and Insect Pests,Nanning 530007,China)

机构地区：[1]广西壮族自治区农业科学院植物保护研究所,南宁530007 [2]农业农村部华南果蔬绿色防控重点实验室,南宁530007 [3]广西作物病虫害生物学重点实验室,南宁530007

出　　处：《应用昆虫学报》2022年第5期1048-1058,共11页Chinese Journal of Applied Entomology

基　　金：广西自然科学基金项目(2018GXNSFBA281155,2021GXNSFAA196059);国家现代农业产业技术体系广西创新团队建设项目(nycytxgxcxtd-2021-01-05);广西农业科学院基本科研业务专项项目(桂农科2021YT073);广西农业科学院科技发展基金项目(桂农科2017JM30,2015JZ44,2015JZ45,2015JZ46)。

摘　　要：【目的】为明确南方水稻黑条矮缩病病毒(Southern rice blackstreaked dwarf virus,SRBSDV)对抗白背飞虱水稻的抗虫性的影响。【方法】在水稻3叶期,每稻苗接入8头1-2龄白背飞虱Sogatella furcifera(Horváth)若虫,参照国际标准评价不同水稻对白背飞虱的抗性表现;每稻苗接入已饲毒且度过循回期的2头白背飞虱高龄若虫,评价不同抗性水平的抗白背飞虱水稻对SRBSDV的抗性。在各水稻成株期分别将不同虫态白背飞虱接到SRBSDV感染的稻株,研究不同抗性水平水稻感染SRBSDV后对白背飞虱取食选择性、取食量、存活率以及产卵量与卵孵化率的影响【结果】中抗、抗和高抗白背飞虱的水稻对SRBSDV的抗性均表现高感,且发病率均在93.55%以上;把无毒白背飞虱若虫分别接入各抗白背飞虱水稻的健康株和SRBSDV病株,48h后在健康株上取食的虫量为1.33-5.33头/株,而在SRBSDV病株上取食的虫量为24.00-46.00头/株,无毒白背飞虱取食选择偏向于SRBSDV病株,且其趋性与水稻对白背飞虱的抗性表现之间无明显相关性;白背飞虱成虫在各水稻健康株和感染SRBSDV的病株上取食,48h后其平均蜜露量分别为1.13-9.55 mg/头和2.97-17.35 mg/头,各抗白背飞虱水稻的感染SRBSDV病株之间白背飞虱的平均蜜露量差异较大,但均显著多于各自健康株上的平均蜜露量;白背飞虱在表现高抗和抗水平水稻上的存活率在15%-44.05%之间,而在感染SRBSDV病株上的存活率显著提高,均在69.21%以上;白背飞虱在各感染SRBSDV抗白背飞虱水稻上无毒白背飞虱能正常产卵及繁殖,且其产卵量和卵孵化率显著多于或高于各自对应健康株。【结论】抗白背飞虱的水稻不一定抗SRBSDV;抗白背飞虱水稻感染SRBSDV,有利于提高白背飞虱在取食、存活和繁殖方面对水稻抗虫性的适应度。[Objectives]To detect the effects of the southern rice black-streaked dwarf virus(SRBSDV)on the resistance of white-backed planthopper(WBPH)resistant rice varieties to the WBPH.[Methods]81st-2nd instar WBPH nymphs were placed on individual rice seedlings to evaluate the resistance of different rice varieties to WBPH,according to the international standard.Each seedling was infected with 2 older nymphs that had been fed with the virus and had passed the cycle period to evaluate the resistance of WBPH-resistant rice varieties to SRBSDV.Different life-stages of WBPH were placed on SRBSDV-infected rice to study the effects of SRBSDV-infection of WBPH-resistant,rice varieties on the feeding selectivity,feeding capacity,survival rate,fecundity and hatchability of WBPH.[Results]Rice varieties with moderate,resistance and high resistance to WBPH were highly susceptible to SRBSDV,with an incidence rate of more than 93.55%.Non-virus WBPH nymphs were inoculated onto both healthy and SRBSDV-infected,WBPH-resistant rice plants.After 48 hours,healthy plants had 1.33-5.33 nymphs/plant whereas SRBSDV-infected plants had 24.00-46.00 nymphs/plant.WBPH prefered to feed on SRBSDV-infected plants,but there was no significant correlation with the resistance of rice to WBPH.After WBPH adults had fed on healthy and SRBSDV-infected rice plants for 48 hours,the average amount of honeydew was 1.13-9.55 mg/adult and 2.97-17.35 mg/adult,respectively.The average amount of honeydew secreted by WBPH feed on SRBSDV-infected,WBPH-resistant,rice varieties was significantly different and higher than that healthy plants.The survival rate of WBPHs on rice with resistance or high resistance was between 15%-44.05%,whereas their survival rate on plants infected with SRBSDV was significantly higher;>69.21%.The non-virus WBPH can reproduce normally on on WBPH-resistant rice varieties that are infected with SRBSDV,and egg production and egg hatching rate are significantly higher on infected plants than on healthy plants.[Conclusion]SRBSDV infection of WBPH

关 键 词：南方水稻黑条矮缩病病毒 白背飞虱 水稻品种 抗虫性 取食量 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]