不同密度黑尾叶蝉取食不同时长后水稻生理生化指标的响应  

Biochemical and physiological response of rice plants induced by the feeding of Nephotettix cincticeps

作　　者：赵文华 杨光梅 刘雨芳[1] ZHAO Wen-Hua;YANG Guang-Mei;LIU Yu-Fang(College of Life Science and Health,Hunan University of Science and Technology,Xiangtan 411201,China)

机构地区：[1]湖南科技大学生命科学与健康学院,湘潭411201

出　　处：《应用昆虫学报》2022年第5期1068-1076,共9页Chinese Journal of Applied Entomology

基　　金：国家重点研发计划(2017YFD0200400)。

摘　　要：【目的】探索不同密度黑尾叶蝉Nephotettix cincticeps取食不同时长后水稻Oryza sativa L.重要生理生化指标的变化。【方法】设置黑尾叶蝉密度为0(对照)、2、4和8头/株4个处理,分别取食6、24、48和96h后,检测水稻叶绿素(Chlorophyll,Chl)与总蛋白(Totalprotein,TP)含量,比较不同取食时间后,水稻脂氧合酶(Lipoxygenase,LOX)、总超氧化物歧化酶(Total superoxide dismutase,T-SOD)、过氧化物酶(Peroxidase,POD)和多酚氧化酶(Polyphenol oxidase,PPO)活性。【结果】不同密度的黑尾叶蝉取食不同时长的水稻后,均极显著地降低水稻叶片的叶绿素含量(P<0.01);取食6h后,水稻叶片的蛋白质含量升高,且随着取食时间的延长,蛋白质含量显著降低(P<0.05)。不同密度的黑尾叶蝉取食后,总体趋势是显著降低水稻LOX活性,但密度为2头/株的黑尾叶蝉取食6h后,引起水稻LOX活性升高。不同密度的黑尾叶蝉取食24h,均引起水稻PPO活性显著升高(P<0.05)。水稻T-SOD活性随着黑尾叶蝉取食胁迫时间呈现动态变化,且与密度有关。不同密度的黑尾叶蝉取食刺激,导致水稻POD活性升高,黑尾叶蝉密度越高,POD活性升高越快,且密度为2头/株和4头/株的黑尾叶蝉取食96 h后,水稻POD活性极显著升高(P<0.01)。【结论】不同密度的黑尾叶蝉取食不同时长,均引起水稻产生明显的生理生化响应,表现为水稻Chl含量及LOX、PPO、T-SOD和POD酶活性的明显变化。[Objectives]To explore the physiological and biochemical responses of rice to the feeding activity of the green rice leafhopper(GRL),Nephotettix cincticeps(Uhler)(Hemiptera:Cicadellidae).[Methods]Physiological and biochemical indices of rice plants were measured at three GRL densities(2,4,and 8 GRLs per plant).Rice Chl and TP content,LOX,T-SOD,POD and PPO activities were measured after GRLs had fed on plants for 6,24,48 and 96 h.The control group was comprised of GRL-free plants.[Results]Chl content of all three density treatments was significantly lower than that of the control group(P<0.01).TP content increased after 6 hours of feeding,then significantly decreased as the duration of feeding increased(P<0.05).LOX activity in the 2 GRLs per plant treatment group significantly increased after 6 hours of feeding(P<0.05),then significantly decreased as the duration of feeding increased.PPO activity significantly increased after 24 hours of feeding in all three density treatment groups(P<0.05).T-SOD activity changed dynamically with the duration of feeding and GRL density.POD activity increased earlier at higher GRL densities;POD activity of the 2 and 4 GRLs per plant treatment groups was significantly higher than that of the control after 96 hours(P<0.051).[Conclusion]Significant physiological and biochemical responses,including changes in Chl content,and in LOX,PPO,T-SOD and POD activity,were induced in rice plants by the feeding activity of N.cincticeps.These responses varied with GRL density and the duration of feeding time.

关 键 词：黑尾叶蝉 取食胁迫 叶绿素 总蛋白 防御蛋白酶 

分 类 号：S435.112.9[农业科学—农业昆虫与害虫防治]