硝基油酸经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞的抗氧化损伤作用  

Antioxidant Effects of Nitro-oleic Acid on Radiation-damaged Rat Submandibular Gland Epithelial Cells via Nrf2/ HO-1 Pathway

作　　者：骆勤亮 张霓霓 龙远铸 柏宇 代敏 唐建宏 崔田宁 黄桂林 LUO Qinliang;ZHANG Nini;LONG Yuanzhu;BAI Yu;DAI Min;TANG Jianhong;CUI Tianning;HUANG Guilin(Department of Oral and Maxillofacial Surgery,Stomatological Hospital Affiliated to Zunyi Medical University,Zunyi 563000,China;Department of Stomatology,The Fifth Affiliated Zhuhai Hospital of Zunyi Medical University,Zhuhai 519110,China)

机构地区：[1]遵义医科大学附属口腔医院口腔颌面外科,贵州遵义563000 [2]遵义医科大学第五附属珠海医院口腔科,广东珠海519110

出　　处：《口腔医学研究》2022年第12期1139-1144,共6页Journal of Oral Science Research

基　　金：国家自然科学基金地区科学基金项目:(项目批准号:81960204);贵州省教育厅青年科技人才成长项目(编号:黔教合KY[2022]291号);遵义市科技计划项目合同书[编号:遵义科核HZ字(2020)298号];遵义医科大学“未来临床名医”项目(编号:20211017)。

摘　　要：目的:硝基油酸(Nitro-oleic acid,OA-NO2)能否经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞发挥抗氧化损伤作用。方法:体外培养乳鼠3 d颌下腺上皮细胞经电子直线加速器建立放射性损伤模型,CCK-8法筛选OA-NO2作用24 h对放射性损伤细胞模型最佳增殖浓度,按实验目的分为正常细胞组(NG)、放射细胞组(RG)、放射细胞+硝基油酸组(R+ONG)、放射细胞+硝基油酸+抑制剂组(R+ON+BRG)、放射细胞+抑制剂组(R+BRG);各组细胞培养24 h后Western blot检测Nrf2、HO-1及NQO1蛋白的表达及活性氧试剂检测活性氧的产生。结果:4μmol/L OA-NO2作用24 h对放射性损伤颌下腺上皮细胞增殖率促进作用最佳;各组细胞培养24 h后,与RG组比较,R+ONG组中Nrf2、HO-1、NQO1蛋白表达明显升高,活性氧的产生明显降低(P<0.05);与R+ONG组比较,R+ON+BRG组中Nrf2、HO-1、NQO1蛋白表达明显降低,活性氧的产生明显升高(P<0.05)。结论:OA-NO2可能经Nrf2/HO-1通路对放射性损伤大鼠颌下腺上皮细胞发挥抗氧化损伤作用。Objective:To detect whether nitro-oleic acid(OA-NO2)can exert antioxidant effect on radiation-damaged rat submandibular gland epithelial cells through Nrf2/HO-1 pathway.Methods:The submandibular gland epithelial cells of rats cultured in vitro for 3 days were established by electron linear accelerator,and the CCK-8 method was used to screen the best proliferation concentration of the radial injury cell model OA-NO2 after 24 h.According to the experimental purpose,they were divided into normal cell group(NG),radioactive cell group(RG),radioactive cell+nitro-oleic acid group(R+ONG),radiation cells+nitro-oleic acid+inhibitor group(R+ON+BRG),and radiation cells+inhibitor group(R+BRG).After 24 h of cell culture,the expressions of Nrf2,HO-1,and NQO1 protein were detected by Western blot,the production of reactive oxygen species was detected by reactive oxygen species reagent,and the proliferation rate was detected by CCK-8 method.Results:The CCK-8 results showed that:4μmol/L OA-NO2 effect for 24 h promoted the proliferation rate of radial injury submandibular gland epithelial cells best.After 24h of cell culture,in contrast to RG group,the expression of Nrf2,HO-1,and NQO1 proteins in R+ONG group increased significantly,and the production of reactive oxygen species decreased significantly(P<0.05).Compared with R+ONG,the expression of Nrf2,HO-1,and NQO1 protein in R+ON+BRG was significantly reduced and the production of reactive oxygen species was significantly increased(P<0.05).Conclusion:Nitro-oleic acid may exert antioxidant effect on rat submandibular gland epithelial cells after radiation injury through Nrf2/HO-1 pathway.

关 键 词：硝基油酸 Nrf2/HO-1通路 颌下腺上皮细胞 放射性损伤 抗氧化损伤 

分 类 号：R818.05[医药卫生—放射医学]