中药冰片对足底切口痛大鼠的镇痛作用研究  

Analgesic effect of borneol on plantar incisional pain in rats

作　　者：黄小琼[1] 刘月姝[1] 王凤姬 冯学轩 郑玉连 代路路 王弋[1] 楼彩霞[1] Huang Xiaoqiong;Liu Yueshu;Wang Fengji;Feng Xuexuan;Zheng Yulian;Dai Lulu;Wang Yi;Lou Caixia(Guangdong Medical Laboratory Animal Center,Guangdong Foshan 528248)

机构地区：[1]广东省医学实验动物中心,广东佛山528248

出　　处：《现代畜牧兽医》2022年第10期11-14,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：广东省中医药管理局课题(20191047,20191046)。

摘　　要：试验旨在研究中药冰片对足底切口痛大鼠的镇痛作用,为实验动物使用与管理委员会(IACUC)福利伦理审查和动物术后镇痛用药选择提供参考。将50只大鼠随机分为假手术组、模型组、复方利多卡因乳膏组以及中药冰片低、高剂量组,每组10只大鼠。建立足底切口痛大鼠模型,术后连续3 d给药,中药冰片低、高剂量组和复方利多卡因乳膏组分别局部表面给予4%、8%中药冰片溶液以及复方利多卡因乳膏,每天2次,单次3 s,间隔约1 h;假手术组、模型组局部表面给予等体积溶媒。末次给药1 h后,测定大鼠的热痛阈值,试验结束测定血常规和血清生化等指标。结果显示:术后2、24和36 h,假手术组、复方利多卡因乳膏组和中药冰片高剂量组的热痛阈值均显著高于模型组(P<0.05)。与模型组相比,术前各组热痛阈值及术后各组大鼠血常规和血清生化指标差异均不显著(P>0.05)。研究表明,中药冰片可提高足底切口痛大鼠术后热痛阈值,有效缓解足底切口痛大鼠的术后疼痛,对大鼠血常规和血生化未见明显影响,可作为动物试验术后局部镇痛的药物选择。The purpose of the experiment was to study the analgesic effect of traditional Chinese medicine borneol on rats with plantar incision pain,and to provide reference for the IACUC welfare ethics review and the selection of postoperative analgesic drugs for animals.A total of 50 rats were randomly divided into sham operation group,model group,compound lidocaine cream group,traditional Chinese medicine borneol low-dose group and high-dose group,with 10 rats in each group.The rat models of planar incision pain were established,and the drugs were administered for three consecutive days after operation.Borneol with low-dose group,borneol with high-dose group and compound lidocaine cream group were locally superficied with 4%,8% Chinese borneol solution and compound lidocaine cream respectively,infiltrate twice a day for 3 s and 1 h at intervals,while sham operation group and model group were locally infiltrated with constant volume solvent.The thresholds of heat pain were determined 1 h after the last infiltration,and the blood routine and serum biochemical indicators were determined at the end of the test.The results showed that at 2,24 and 36 h after operation,the thermal pain thresholds in sham operation group,compound lidocaine cream group and borneol with high-dose group were significantly higher than those in model group(P<0.05).Compared with model group,there were no significant differences in the preoperative thermal pain threshold and postoperative blood routine and serum biochemical indexes in each group(P>0.05).The study indicates that traditional Chinese medicine borneol can improve the postoperative thermal pain threshold in rats with plantar incision pain,effectively relieve postoperative pain in rats with plantar incision pain,and has no obvious effect on blood routine and blood biochemistry in rats,can be used as a drug of choice for local analgesia after animal experiments.

关 键 词：中药冰片 热痛阈值 足底切口痛大鼠模型 

分 类 号：S859[农业科学—临床兽医学]