脓毒症急性肾损伤患者血清miR-125b-5p的表达水平及意义  

Expression level and significance of serum miR-125b-5p in septic acute kidney injury patients

作　　者：陶伍元 邓柳霞[2] 李晓萍 Tao Wuyuan;Deng Liuxia;Li Xiaoping(Department of Emergency,Shenzhen Bao'an District People's Hospital,Shenzhen 518101,Guangdong,China;Department of Nephrology,Shenzhen Bao'an District People's Hospital,Shenzhen 518101,Guangdong,China)

机构地区：[1]深圳市宝安区人民医院急诊科,广东深圳518101 [2]深圳市宝安区人民医院肾内科,广东深圳518101

出　　处：《实用检验医师杂志》2022年第3期299-302,共4页Chinese Journal of Clinical Pathologist

基　　金：深圳市宝安区科技计划项目(2019JD032)。

摘　　要：目的探讨miR-125b-5p在脓毒症急性肾损伤(SAKI)患者血清中的表达水平及其与血管内皮钙黏蛋白(VE-cadherin)的相关性。方法选择2019年6月—2021年12月深圳市宝安区人民医院收治的38例SAKI患者作为SAKI组,另外选择40名同期健康体检者作为对照组。采集所有受检者静脉血标本,对各组中3例患者的血清样本进行微小RNA(miRNA)测序分析;采用实时荧光定量聚合酶链反应(PCR)检测两组血清miR-125b-5p表达;进行京都基因和基因组数据库(KEGG)富集分析;采用酶联免疫吸附试验(ELISA)检测血清中VE-cadherin水平;采用Pearson相关分析法对血清miR-125b-5p表达与VE-cadherin水平进行相关性分析。结果①实时荧光定量PCR结果显示,SAKI组的miR-125b-5p表达水平明显高于对照组(3.65±0.39比1.25±0.21,P<0.05)。②KEGG信号通路富集分析结果显示,靶基因富集于乳头瘤病毒感染、磷脂酰肌醇3-激酶-蛋白激酶B(I3K-Akt)信号通路、肌动蛋白骨架调节、丝裂原活化蛋白激酶(MAPK)信号通路。③SAKI组患者血清VE-cadherin水平明显高于对照组(mg/L:22.39±5.24比15.54±4.78,P<0.05)。④相关性分析显示,miR-125b-5p表达水平与血清中VE-cadherin水平呈正相关(r值为0.743,P值为0.001)。结论SAKI患者血清miR-125b-5p表达水平与血清中的VE-cadherin水平呈正相关,两者间可能存在一定的调控关系。Objective To investigate the expression level of miR-125b-5p in serum of septic acute kidney injury(SAKI)patients and its relationship with vascular endothelial cadherin(VE-cadherin).Methods The 38 sepsis patients admitted to Shenzhen Bao'an District People's Hospital from June 2019 to December 2021 were selected as SAKI group,and 40 health examinees in the same period were selected as control group.The venous blood samples of all subjects were collected,and three patients'sera in each group were selected for microRNA(miRNA)sequencing analysis.The expression of miR-125b-5p in serum of two groups was detected by real-time fluorescence quantitative polymerase chain reaction(PCR).The enrichment analysis of Kyoto Gene and Genome Database(KEGG)was carried out.Enzyme linked immunosorbent assay(ELISA)was used to detect the level of VE-cadherin in serum.The correlation between serum miR-125b-5p and VE-cadherin levels was analyzed.Results①Real-time quantitative PCR detection showed that compared with control group,the level of miR-125b-5p expression in SAKI group was elevated(3.65±0.39 vs.1.25±0.21,P<0.05).②The target genes were enriched in human papillomavirus infection,PI3K-Akt signaling pathway,regulation of actin cytokeleton and MAPK signaling pathway in KEGG enrichment analysis.③The serum VE-cadherin level of SAKI patients was higher than that in control group(mg/L:22.39±5.24 vs.15.54±4.78,P<0.05).④miR-125b-5p expression level was positively correlated with serum VE-cadherin level(r value was 0.743 and P value was 0.001).Conclusion The expression level of serum miR-125b-5p in SAKI patients is positively correlated with serum VE-cadherin level,with a certain regulatory relationship.

关 键 词：miR-125b-5p 脓毒症 急性肾损伤 血管内皮钙黏蛋白 

分 类 号：R459.7[医药卫生—急诊医学] R692[医药卫生—治疗学]