2018-2020年广州急性呼吸道感染患儿中腺病毒分子流行病学特征研究  

Molecular Epidemiological Characteristics of Adenoviruses in Children with Acute Respiratory Tract Infection in Guangzhou,China,from 2018 to 2020

作　　者：周吉剑 李万维 于博 王烟岚 薛豹 李弘剑[1] ZHOU Jijian;LI Wanwei;YU Bo;WANG Yanlan;XUE Bao;LI Hongjian(College of Life Science and Technology of Jinan University,The Key Laboratory for Virology,Guangzhou 510632,China)

机构地区：[1]暨南大学生命科学技术学院病毒学重点实验室,广州510632

出　　处：《病毒学报》2022年第6期1346-1355,共10页Chinese Journal of Virology

基　　金：“十三五”国家传染病防治重大专项(项目号:2017ZX10103011-007),题目:珠三角地区儿童传染病重要症候群病原谱及危害重症病原特征研究。

摘　　要：为了解2018-2020年间广州地区急性呼吸道感染儿童中腺病毒的流行病学特征,收集广东省妇幼保健院番禺院区和暨南大学附属第一医院儿科住院部急性呼吸道感染患儿咽拭子标本,采用荧光定量PCR技术检测腺病毒核酸,分析腺病毒的流行病学特征。所有腺病毒阳性标本接种A549细胞进行病毒分离,获得临床分离株后,对不同型别分离株的生长特点进行研究,并比较了感染患儿的临床特征。结果显示,在收集的701份急性呼吸道感染患儿的咽拭子标本中,腺病毒阳性率为4.99%(35/701),易感人群为5岁以下儿童。系统发育分析显示主要型别为B种3型腺病毒(42.86%)与B种7型腺病毒(14.29%)。经细胞培养后获得腺病毒阳性毒株28株,分离率为80%。临床分离株感染特征分析结果显示,PCR检测B种7型腺病毒分离株病毒基因组拷贝数显著高于其他基因型。对感染患儿临床特征的分析显示,B种3型腺病毒感染患儿中性粒细胞增高伴淋巴细胞降低。上述结果表明,2018-2020年间急性呼吸道感染的住院儿童腺病毒阳性患儿中,B种3型与B种7型为优势流行基因型,病毒基因进化高度保守。临床分离株中,B种7型腺病毒生长速率较高。To explore the epidemiological characteristics of adenoviruses in children with acute respiratory tract infection(ARTI)in Guangzhou,China,from 2018 to 2020.The nasopharyngeal(NP)swabs of children with ARTI collected from Guangdong Panyu Maternity and Children’s Health Care Hospital and The First Affiliated Hospital of Jinan University were tested by quantitative polymerase chain reactions to measure adenovirus load.Clinical isolates were obtained by inoculating into cell culture,their growth characteristics studied,and the clinical characteristics of infected children documented.The percentage of HAdV-positive samples was 4.99%in 701 NP(35/701)swabs collected.The susceptible population was children under 5years of age.Phylogenetic analyses showed that the main genotypes were B-species HAdV-3(42.86%)and HAdV-7(14.29%).Twenty-eight cell cultures with sample inoculation were amplified and found to be positive for adenovirus DNA.Of all the genotypes obtained,HAdV-7 had the most copies of the viral genome.Analyses of the clinical characteristics of infected children showed that HAdV-3 was prone to increase the number of neutrophils and lymphocytopenia.Our study suggested that HAdV-3 and HAdV-7 were the predominant genotypes in hospitalized children with an ARTI in Guangzhou,China.Viral genes had highly conserved evolution.Among all the clinical isolates,HAdV-7 has high growth rate.

关 键 词：人腺病毒 急性呼吸道感染 HEXON Fiber 

分 类 号：R511.8[医药卫生—内科学] R373.1[医药卫生—临床医学]