烟台沿海地区贝类海产品戊型肝炎病毒检测分析  

Investigation of Hepatitis E Virus Contamination in Shellfish Seafood in Coastal Area of Yantai,China

作　　者：崔伟红 刘娟[2] 孙振璐[2] 高巧[2] 董兆静 宫连凤 马莉[3] CUI Weihong;LIU Juan;SUN Zhenlu;GAO Qiao;DONG Zhaojing;GONG Lianfeng;MA Li(Shandong College of Traditional Chinese Medicine,Yantai 264003,China;Yantai Center for Disease Prevention and Control,Yantai 264003,China;ShandongUniversityof Traditional ChineseMedicine,Jinan 250355,China)

机构地区：[1]山东中医药高等专科学校医学系,烟台264003 [2]烟台市疾病预防控制中心微生物检验科,烟台264003 [3]山东中医药大学,济南250355

出　　处：《病毒学报》2022年第6期1391-1396,共6页Chinese Journal of Virology

基　　金：山东省医药卫生科技发展计划项目(项目号:202012050924)。

摘　　要：了解烟台地区贝类海产品中戊型肝炎病毒污染状况,为戊型肝炎防控提供科学依据。采集了烟台近海区域贝类海产品11种,用PEG 8000对戊肝病毒进行优化富集,应用实时荧光定量RT-PCR方法进行检测,用已知戊肝阳性病人粪便标本做系列梯度稀释作为荧光信号强度参照。11种共151份贝类海产品中,在海虹(贻贝)中检出戊肝病毒阳性3份,可疑阳性2份,其它10种贝类中未检出。烟台近海区域贝类海产品(贻贝)中存在戊型肝炎病毒污染。To investigate hepatitis E virus(HEV)contamination in coastal area of Yantai region and provide scientific basis for prevention and control of HEV.Eleven species of shellfish were collected in coastal area of Yantai.Hepatitis E virus were enriched with PEG 8,000,then detected by real-time PCR.Positive feacal specimens from hepatitis E patients were serial diluted and used as fluorescence signal reference.A total of 151samples from 11 species shellfishes were tested and hepatitis E virus were detected only from mussles.Among them 3 samples were positive and 2 samples were suspicious.Other shellfishes were negative.The shellfishes(mussles)from coastal area of Yantai were contaminated by hepatitis E virus.

关 键 词：贝类海产品 戊型肝炎病毒 实时荧光定量RT-PCR 

分 类 号：R3733[医药卫生—病原生物学]