加压毛细管电色谱分离氢溴酸樟柳碱等三种生物碱类成分  

Eparation of Three Alkaloids Such as Anisodine Hydrobromide by Pressurized Capillary Electrochromatography

作　　者：张潇晗 张晓蕾 牛丽颖[1,2,3] 毕晓东 ZHANG Xiao-han;ZHANG Xiao-lei;NIU Li-ying;BI Xiao-dong(School of Materia Medica,Hebei University of Chinese Medicine,Hebei Shijiazhuang 050200;Hebei Traditional Chinese Medicine Formula Granule Technology Innovation Center,Hebei Shijiazhuang 050091;Traditional Chinese Medicine Formula Granule Research Center of Hebei Province University,Hebei Shijiazhuang 050091,China)

机构地区：[1]河北中医学院,河北石家庄050091 [2]河北省中药配方颗粒创新中心,河北石家庄050091 [3]河北省中药材品质评价与标准化工程研究中心,河北石家庄050091

出　　处：《广州化工》2022年第21期146-148,共3页GuangZhou Chemical Industry

基　　金：河北中医学院科技能力提升项目(No:KTY2019067);河北中医学院大学生创新创业项目(No:201914432107)。

摘　　要：建立了加压毛细管电色谱法(pCEC)分离氢溴酸樟柳碱、氢溴酸东莨菪碱、氢溴酸山莨菪碱3中生物碱类成分的方法。该方法采用EP-100-20/45-3-C_(18)毛细管色谱柱(总长度45 cm,有效长度20 cm,直径为100μm,ODS填料3μm)进行分离,流动相为乙腈-50 mmol/L磷酸二氢钾(含0.08%三乙胺)(15:85体积比),流动相的流速为0.07 mL/min,分离电压为0 kV,紫外检测波长为210 nm。结果表明,三种生物碱类成分分离良好。A method for the separation of alkaloids from anisodine hydrobromide,scopolamine hydrobromide and anisodamine hydrobromide by pressurized capillary electrochromatography(pCEC)was established.The method adopted EP-100-20/45-3-C_(18) capillary chromatographic column(total length 45 cm,effective length 20 cm,diameter 100μm,ODS packing 3μm).The mobile phase was acetonitrile-50 mmol/L potassium dihydrogen phosphate(containing 0.08%triethylamine)(15:85 volume ratio),the flow rate of the mobile phase was 0.07 mL/min,the separation voltage was 0 kV,and UV detection wavelength was 210 nm.The results showed that the three alkaloids were well separated.

关 键 词：加压毛细管电色谱 生物碱 分离 

分 类 号：O652.7[理学—分析化学]