工业蛋白质理性设计与应用  

Rational design and applications of industrial proteins

作　　者：曲戈 袁波 孙周通 QU Ge;YUAN Bo;SUN Zhoutong(Tianjin Institute of Industrial Biotechnology,Chinese Academy of Science,Tianjin 300308,China;National Technology Innovation Center of Synthetic Biology,Tianjin 300308,China)

机构地区：[1]中国科学院天津工业生物技术研究所,天津300308 [2]国家合成生物技术创新中心,天津300308

出　　处：《生物工程学报》2022年第11期4068-4080,共13页Chinese Journal of Biotechnology

基　　金：天津市合成生物技术创新能力提升行动(TSBICIP-CXRC-009,TSBICIP-CXRC-040);天津市自然科学基金(21JCJQJC00110)。

摘　　要：作为合成生物学与绿色生物制造等领域的底层核心技术,蛋白理性设计可有效解决天然功能元件性能不足等共性挑战,创制高性能人工酶元件。值此天津工业生物研究所(Tianjin Institute of Industrial Biotechnology,TIB)创立10周年之际,文中回顾了研究所在工业蛋白理性设计领域的系列重要工作进展。从酶设计方法学研究、新酶反应设计到生物催化应用等方面进行了分析讨论,并展望了本领域未来发展方向。望借此搭建学术界和产业界与酶理性设计的桥梁,促进新技术、新策略的开发应用,加速融合人工酶的基础研究与产业应用,推动我国生物制造领域的科技创新升级。As one of the underlying core technologies in the fields of synthetic biology and green bio-manufacturing,rational protein design is able to effectively solve generic challenges,e.g.,improving insufficient performance of natural enzymes,and creating high-performance artificial enzymes.On the occasion of the 10th anniversary of the founding of the Tianjin Institute of Industrial Biotechnology(TIB),Chinese Academy of Sciences,this paper reviews the important progress of TIB achieved in rational design of industrial proteins,from the development of enzyme design methodology,the design of new enzyme reactions,to the applications of biocatalysis,and prospects future trends of this field.It is hoped that this will build a bridge between academia and industry on the rational design of enzymes,promote the development and application of new technologies and strategies.This will help merging the basic research and industrial application,thereby advancing the bio-manufacturing technological innovation.

关 键 词：蛋白质工程 理性设计 催化机制 人工酶 生物催化 

分 类 号：Q51[生物学—生物化学]