工业酶与绿色生物工艺的核心技术进展  

Developments of core technologies in industrial enzymes and green bioprocessing

作　　者：郑宏臣[1,2] 徐健勇 杨建花[1,2] 郑迎迎 涂然 石婷 付刚[1,2] 刘倩 王兴彪 韩旭[1,2] 张以恒 柏文琴[1,2] 宋诙 ZHENG Hongchen;XU Jianyong;YANG Jianhua;ZHENG Yingying;TU Ran;SHI Ting;FU Gang;LIU Qian;WANG Xingbiao;HAN Xu;ZHANG Yiheng;BAI Wenqin;SONG Hui(National Engineering Research Center for Industrial Enzymes,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;National Technology Innovation Center of Synthetic Biology,Tianjin 300308,China)

机构地区：[1]中国科学院天津工业生物技术研究所工业酶国家工程研究中心,天津300308 [2]国家合成生物技术创新中心,天津300308

出　　处：《生物工程学报》2022年第11期4219-4239,共21页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2021YFC2100201,2021YFC2100405,2021YFC2100403);天津市合成生物技术创新能力提升行动(TSBICIP-KJGG-009-0202)。

摘　　要：绿色生物工艺是国家战略性新兴产业,具备高效、安全、节能、环保等特点,市场前景广阔。工业酶是绿色生物工艺的“芯片”,新型高效工业酶的开发与应用相关技术是绿色生物工艺的核心。文章综述了工业酶制剂产业现状、以中国科学院天津工业生物技术研究所为代表的研发机构在工业酶及绿色生物工艺开发应用方面产生的一系列关键技术突破与研发进展,展示了通过酶与生物技术的进步提升传统加工产业发展的典型案例,随着这些核心技术的发展将推动越来越多的传统制造业走向绿色可持续发展路线。The green bio-manufacturing industry,characterized with high efficiency,safety,energy-saving,and environmental-friendliness,is a national strategic emerging industry with broad market prospect.Industrial enzyme is the“chip”of green biological process.The exploitation and application of new industrial enzymes is one of the core enabling technologies of green bio-manufacturing.This review introduces the current situation of industrial enzyme industry,followed by summarizing a series of key technical breakthroughs and research progress in industrial enzymes as well as green biological technologies and processes,which were developed by Tianjin institute of industrial biotechnology,Chinese Academy of Sciences in the past 10 years.Typical cases where traditional processing industry was promoted by the development and application of enzyme and green biological technologies were also presented.It is envisioned that development of these core technologies will enable more traditional processing industries transform into green and sustainable bio-based industry.

关 键 词：工业酶 基因挖掘 高通量筛选 蛋白理性设计 定向进化 表达系统 绿色生物加工 

分 类 号：Q814[生物学—生物工程] TQ426.97[化学工程]