尖锐湿疣患者皮损组织中Smac、BIRC5的表达及意义  

Expression and clinical significance of Smac and BIRC5 in the skin lesions of condyloma acuminatum patients

作　　者：牛理达 毕新岭[2] 张瑞 丁菲[1] NIU Li-da;BI Xin-ling;ZHANG Rui;DING Fei(Department of Dermatological,Zhoukou Central Hospital,Zhoukou 466000,Henan,China)

机构地区：[1]周口市中心医院皮肤科,河南周口466000 [2]上海长海医院皮肤科,上海200433

出　　处：《广东医学》2022年第9期1124-1129,共6页Guangdong Medical Journal

基　　金：上海市浦江人才计划项目(18PJD053)。

摘　　要：目的 探讨尖锐湿疣(CA)组织中第二个线粒体衍生半胱天冬氨酸蛋白酶激活剂(Smac)和凋亡抑制因子5(BIRC5)的表达及其临床意义。方法 选取100例CA患者作为研究对象并设置为研究组,另选择同时期103例非皮肤病行手术切除后留取切缘处正常皮肤组织的患者为对照组;采用免疫组织化学法检测组织中Smac、BIRC5的表达情况;采用实时荧光定量PCR(qRT-PCR)检测组织中Smac mRNA、BIRC5 mRNA表达水平;采用Spearman相关性分析Smac、BIRC5的相关性;使用ROC曲线分析Smac、BIRC5对CA的诊断价值;多因素logistic回归分析影响CA发生的危险因素。结果 研究组CA组织中BIRC5阳性率、相对表达量高于对照组,Smac阳性率、相对表达量低于对照组(P<0.05);CA组织中Smac、BIRC5表达与病程、HPV感染类型、复发次数密切相关(P<0.05);CA组织中Smac、BIRC5表达呈负相关(P<0.05);ROC曲线分析结果表明,与Smac、BIRC5单独相比,两者联合诊断CA发生的AUC更高(z=3.886、3.564,P均=0.000);多因素logistic回归分析显示,Smac阴性表达、BIRC5阳性表达是影响CA发生的危险因素(P<0.05)。结论 Smac、BIRC5在CA组织中表达异常,检测两者水平对CA的早期防治具有重要意义。Objective To investigate the expression and clinical significance of the second mitochondrial-derived activator of caspase(Smac)and baculoviral IAP repeat-containing protein 5(BIRC5)in condyloma acuminatum(CA)tissue.Methods One hundred CA patients were regarded as research group.In addition,103 patients with non-dermatological diseases during the same course who underwent surgical resection and left normal skin tissue at the incision margin were gathered as the control group.Immunohistochemistry was performed to measure the expression of Smac and BIRC5 in tissues;Real-time fluorescent quantitative PCR(qRT-PCR)was performed to measure the expression levels of Smac mRNA and BIRC5 mRNA in the tissues;Spearman correlation was performed to analyze the correlation between Smac and BIRC5;ROC curve was performed to analyze the diagnostic value of Smac and BIRC5 for CA;and multivariate logistic regression was performed to analyze the risk factors affecting the occurrence of CA.Results The positive rate and relative expression of BIRC5 in CA tissues in the study group were higher than those in the control group,and the positive rate and relative expression of Smac were lower than those in the control group(P<0.05).The expression of Smac and BIRC5 in CA tissues was closely correlated to the course of disease,HPV infection type,and the times of recurrence(P<0.05).The expression of Smac and BIRC5 in CA tissues was significantly negatively correlated(P<0.05).ROC curve analysis results showed that compared with Smac and BIRC5 alone,the AUC of the two combined diagnosis of CA was significantly higher(z=3.886,3.564,both P=0.000).Multivariate logistic regression analysis showed that negative expression of Smac and positive expression of BIRC5 were risk factors affecting the occurrence of CA(P<0.05).Conclusion Smac and BIRC5 are abnormally expressed in CA tissues,and the detection of their levels is of great significance for the early prevention and treatment of CA.

关 键 词：尖锐湿疣 第二个线粒体衍生半胱天冬氨酸蛋白酶激活剂 凋亡抑制因子5 

分 类 号：R752.53[医药卫生—皮肤病学与性病学] R446[医药卫生—临床医学]