基于金属有机骨架衍生的CuO纳米酶比色法检测多巴胺  

Colorimetric Detection of Dopamine Based on Metal-Organic Framework-Derived CuO Nanozymes

作　　者：冯珣 范荣华 邓丽丽[1] 陈阳 Feng Xun;Fan Ronghua;Deng Lili;Chen Yang(School of Public Health,Shenyang Medical College,Shenyang,110034;School of Pharmacy,China Medical University,Shenyang,110122)

机构地区：[1]沈阳医学院公共卫生学院,沈阳110034 [2]中国医科大学药学院,沈阳110122

出　　处：《化学通报》2022年第11期1363-1370,共8页Chemistry

基　　金：辽宁省自然科学基金项目(2020-MS-310)资助2022-04-04,2022-05-09。

摘　　要：本文通过简单热裂解由硝酸铜和1,3,5-苯三酸合成的铜基有机骨架材料制备了一种CuO纳米材料。利用扫描电镜、透射电镜、X射线衍射及X射线光电子能谱对CuO的结构及元素组成进行了表征。该CuO纳米材料表现出良好的过氧化物酶活性,在H_(2)O_(2)存在下,可以催化氧化无色底物3,3′,5,5′-四甲基联苯胺(TMB)变成蓝色产物。选择对苯二甲酸作为荧光探针验证了CuO的类酶催化机制,结果表明,CuO会催化H_(2)O_(2)产生·OH,其可氧化TMB形成蓝色产物,而多巴胺的存在会抑制CuO对H_(2)O_(2)氧化TMB的催化活性,从而实现对多巴胺的检测。该方法对多巴胺的检测具有良好的稳定性和催化活性,线性范围为5~50μmol/L和50~200μmol/L,检测限为0.5μmol/L。该比色传感器可以用于对人体尿液中的多巴胺的检测,表明该CuO纳米酶在生物和临床诊断方面具有潜在的应用价值。In this paper, CuO nanomaterial were synthesized by simple pyrolysis of Cu-based organic framework material(Cu-MOF) synthesized from copper nitrate and 1,3,5-benzenetricarboxylic acid. The structure and elemental composition of CuO were characterized by SEM, TEM, XRD and XPS. The CuO nanomaterial exhibits good peroxidase-like activity and can catalyze the oxidation of colorless substrate 3,3′,5,5′-tetramethylbenzidine(TMB) to blue product in the presence of hydrogen peroxide(H_(2)O_(2)). The enzyme-like catalytic mechanism of CuO was studied by fluorometry using terephthalic acid as fluorescent probe. The result showed that CuO can catalyze H_(2)O_(2)to produce hydroxyl radical, and hydroxyl radical can oxidize TMB to form blue product. Oxidative TMB discoloration of CuO in the presence of H_(2)O_(2)can provide an effective platform for colorimetric detection of dopamine. The presence of dopamine can inhibit the catalytic activity of CuO for the oxidation of TMB by H_(2)O_(2), thus realizing the detection of dopamine. This method had good stability and catalytic activity for the detection of dopamine. The linear range is 5~50 μmol/L and 50~200 μmol/L, and the detection limit is 0.5 μmol/L. This colorimetric sensor can be used for the detection of dopamine in human urine, indicating that CuO nanozyme has potential application value in biological and diagnostic applications.

关 键 词：氧化铜 纳米酶 比色分析 多巴胺 

分 类 号：R313[医药卫生—基础医学] O657.3[理学—分析化学]