两株虫生真菌对草地贪夜蛾的致病力及高毒力菌株与卵寄生蜂的相容性  

Pathogenicity of two entomopathogenic fungi against the fall armyworm Spodoptera frugiperda and their compatibility with egg parasitoids

作　　者：苟雪莲 王振营[2] 涂雄兵[2] 龙秀珍[3] 于永浩[3] 施建琴 郭井菲[2] 董辉[1] Gou Xuelian;Wang Zhenying;Tu Xiongbing;Long Xiuzhen;Yu Yonghao;Shi Jianqin;Guo Jingfei;Dong Hui(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,Liaoning Province,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Guangxi Key Laboratory for Biology of Crop Diseases and Insect Pests,Key Laboratory of Green Prevention and Control of Fruit and Vegetable Pest in South China,Ministry of Agriculture and Rural Affairs,Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]广西壮族自治区农业科学院植物保护研究所,农业农村部华南果蔬绿色防控重点实验室,广西作物病虫害生物学重点实验室,南宁530007

出　　处：《植物保护学报》2022年第5期1505-1512,共8页Journal of Plant Protection

基　　金：国家重点研发计划(2021YFD1400705);中国农业科学院科技创新工程(CAAS-ZDRW202108)。

摘　　要：为有效防控新入侵我国的重大农业害虫草地贪夜蛾Spodoptera frugiperda,采用浸叶法测定球孢白僵菌Beauveria bassiana菌株Bb1237、Bb201017、Bb20091317及金龟子绿僵菌Metarhizium anisopliae菌株Ma189和莱氏绿僵菌M.rileyi菌株MrCDTLJ1对草地贪夜蛾3龄幼虫的致病力,筛选对草地贪夜蛾3龄幼虫有较强致病力的菌株,并测定该菌株对草地贪夜蛾3龄幼虫的致病力和对其天敌的致死率。结果显示,球孢白僵菌Bb20091317和莱氏绿僵菌MrCDTLJ1对草地贪夜蛾3龄幼虫的累计校正死亡率显著高于其他菌株,分别为95.59%和97.06%,致死中时LT50分别为3.42 d和5.72 d,LC_(50)分别为7.05×10^(6)个/mL和2.25×10^(5)个/mL。2种菌在高浓度1×10^(8)个/mL下对草地贪夜蛾天敌夜蛾黑卵蜂Telenomus remus和玉米螟赤眼蜂Trichogramma ostriniae成蜂的致死率也仅介于15%~20%之间。表明球孢白僵菌Bb20091317和莱氏绿僵菌MrCDTLJ1不仅对草地贪夜蛾有高致病力,同时与夜蛾黑卵蜂和玉米螟赤眼蜂有较好的相容性。In order to effectively control the fall armyworm Spodoptera frugiperda, an important agricultural pest that has recently invaded China, the pathogenicity of Beauveria bassiana strains Bb1237,Bb201017, Bb20091317, Metarhizium anisopliae strain Ma189 and M. rileyi strain MrCDTLJ1 against the 3rd instar larvae of S. frugiperda were determined by the leaf impregnation method. The pathogenicity of highly pathogenic strains to the 3rd instar larvae of fall armyworm and the mortality of its natural enemies were determined. The results showed that the corrected accumulative mortalities of S. frugiperda 3rd instar larvae caused by B. bassiana Bb20091317 and M. rileyi MrCDTLJ1 were 95.59% and97.06%, respectively, significantly higher than those caused by other strains. The median lethal time(LT) values were 3.42 d, 5.72 d and the lethal concentration(LC_(50)) values were 7.05×10^(6) conidia/mL and 2.25×10^(5) conidia/mL, respectively. The mortalities of the natural enemies of Telenomus remus and Trichogramma ostriniae caused by B. bassiana Bb20091317 and M. rileyi MrCDTLJ1 were 15%-20%at the concentration of 1×10^(8) conidia/mL. The results suggested that B. bassiana Bb20091317 and M. rileyi MrCDTLJ1 were highly pathogenic to S. frugiperda and compatible with T. remus and T. ostriniae.

关 键 词：草地贪夜蛾 虫生真菌 卵寄生蜂 相容性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]