安徽省不同地理种群草地贪夜蛾对Bt蛋白的敏感性  

Susceptibility of different geographical populations of fall armyworm Spodoptera frugiperda in Anhui Province to Bt proteins

作　　者：徐婷婷 王月琴[2] 胡飞 毕思佳 胡本进 王振营[2] 徐丽娜 Xu Tingting;Wang Yueqin;Hu Fei;Bi Sijia;Hu Benjin;Wang Zhenying;Xu Lina(Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,Anhui Province,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护学报》2022年第5期1521-1527,共7页Journal of Plant Protection

基　　金：国家重点研发计划(2021YFD1400700)。

摘　　要：为明确安徽省不同生态区草地贪夜蛾Spodoptera frugiperda田间种群对不同转Bt玉米的敏感性,于2020—2021年采用饲料药膜法比较不同地理种群对Bt蛋白Cry1Ab和Cry1F的敏感性。结果显示,2020年,Cry1F蛋白对草地贪夜蛾各地理种群的活性普遍好于Cry1Ab蛋白,Cry1Ab蛋白对无为、埇桥、太和及长丰种群的LC_(50)分别为537.60、484.84、474.18和93.37 ng/cm^(2),而Cry1F蛋白对这4个种群的LC_(50)分别为274.74、48.52、44.44和10.64 ng/cm^(2),均低于Cry1Ab蛋白;Cry1F蛋白仅对宿松种群的LC_(50)(61.81 ng/cm^(2))略高于Cry1Ab蛋白(LC_(50)为54.97 ng/cm^(2))。2021年,Cry1Ab蛋白对无为、太和及长丰种群的LC_(50)分别为235.62、107.76和26.33 ng/cm^(2),与2020年相比分别下降了56.17%、77.27%和71.80%;而Cry1F蛋白对这3个种群的LC_(50)分别为236.84、76.88和24.85 ng/cm^(2),与2020年相比,Cry1F蛋白对无为种群的LC_(50)下降了13.79%;而对太和及长丰种群的LC_(50)分别上升了73.00%和133.55%。表明Cry1Ab和Cry1F蛋白对草地贪夜蛾各地理种群均具有一定活性,可用于草地贪夜蛾的防治。In order to clarify the susceptibility of fall armyworm Spodoptera frugiperda in different planting areas of Anhui Province to Bt proteins,the artificial diet surface overlay method was used to compare the susceptibility of different geographic populations of S.frugiperda to Bt proteins,Cry1Ab and Cry1F in 2020—2021.The results showed that Cry1F had better insecticidal activity than Cry1Ab against most geographical populations in 2020.The LC_(50) values of Cry1Ab protein to Wuwei,Yongqiao,Taihe and Changfeng populations were 537.60,484.84,474.18,and 93.37 ng/cm^(2),respectively,while the LC_(50) values of Cry1F to these populations were 274.74,48.52,44.44,and 10.64 ng/cm^(2),respectively.The LC_(50) value of Cry1F to Susong population was 61.81 ng/cm^(2),which was slightly higher than that of Cry1Ab(54.97 ng/cm^(2)).In 2021,the LC_(50) values of Cry1Ab protein to Wuwei,Taihe and Changfeng populations were 235.62,107.76 and 26.33 ng/cm^(2),decreased by 56.17%,77.27%and 71.80%compared with 2020,respectively.In 2021,the LC_(50) values of Cry1F to the three populations,were 236.84,76.88 and 24.85 ng/cm^(2),respectively.Compared with the results of 2020,the LC_(50) value of Cry1F to Wuwei population decreased by 13.79%;the LC_(50) values of Taihe and Changfeng populations increased by 73.00%and 133.55%,respectively.This study revealed that Bt proteins,Cry1Ab and Cry1F had activities against various geographic populations of S.frugiperda and could be used for the control of S.frugiperda.

关 键 词：草地贪夜蛾 敏感性 Cry1Ab蛋白 Cry1F蛋白 转Bt玉米 田间种群 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]