自然杀伤T细胞、转录因子RORγt及Foxp3在白癜风患者外周血中的表达水平及其意义  

Expression and Significance of Natural Killer T Cells,Transcription Factor RORγt and Foxp3 in Peripheral Blood of Patients with Vitiligo

作　　者：王小媛[1] 曹伟[1] 王颖娟[1] 王晓晓 WANG Xiao-yuan;CAO Wei;WANG Ying-juan(Department of Dermatology,Baoji Central Hospital,Baoji Shaanxi 721000)

机构地区：[1]宝鸡市中心医院皮肤科,陕西宝鸡721000

出　　处：《医学临床研究》2022年第10期1445-1448,共4页Journal of Clinical Research

基　　金：陕西省科技厅重点研发计划项目(2017SF-153)。

摘　　要：【目的】探讨自然杀伤T细胞(NK-T)、孤独核受体(RORγt)、叉头翼状螺旋转录因子3(Foxp3)在白癜风患者外周血中的表达水平及其意义。【方法】选取2019年4月至2021年5月本院诊治的89例白癜风患者(观察组),另外选取同期在本院体检的36例健康志愿者(对照组)。检测并比较两组研究对象外周血NK-T、RORγt mRNA、Foxp3 mRNA水平;比较不同病期、病型、皮损面积的白癜风患者外周血NK-T、RORγt mRNA、Foxp3 mRNA水平,分析皮损面积与NK-T及RORγt mRNA、Foxp3 mRNA水平相关性。【结果】观察组患者外周血RORγt mRNA水平显著高于对照组,NK-T、Foxp3 mRNA水平显著低于对照组,差异有统计学意义(P<0.05);与稳定期相比,进展期患者RORγt mRNA水平较高,NK-T、Foxp3 mRNA水平较低,差异有统计学意义(P<0.05);不同病型的白癜风患者NK-T、RORγt mRNA、Foxp3 mRNA水平比较,差异有统计学意义(P<0.05);不同皮损面积间NK-T、RORγt mRNA、Foxp3 mRNA水平比较,差异有统计学意义(P<0.05);RORγt mRNA与皮损面积呈正相关(rs=0.509,P<0.05),NK-T、Foxp3 mRNA与皮损面积呈负相关(rs=-0.348、-0.307,P<0.05)。【结论】外周血NK-T及RORγt mRNA、Foxp3 mRNA水平异常与白癜风发病及病情发展密切相关,白癜风患者皮损面积与RORγt mRNA呈正相关,与NK-T、Foxp3 mRNA呈负相关。【Objective】To investigate the expression and significance of natural killer T cells(NK-T),RORγt and Foxp3 in peripheral blood of patients with vitiligo.【Methods】A total of 89 patients with vitiligo diagnosed and treated in our hospital from April 2019 to May 2021(observation group)were selected,and 36 healthy volunteers(control group)who were examined in our hospital at the same time were selected.The levels of NK-T,RORγt mRNA and Foxp3 mRNA in peripheral blood of the two groups of subjects were detected and compared;The levels of peripheral blood NK-T,RORγt mRNA and Foxp3 mRNA of vitiligo patients with different disease stages,disease types and skin lesion areas were compared.Correlation of the skin lesion area,NK-T,RORγt mRNA and Foxp3 mRNA levels were analyzed.【Results】The level of the peripheral blood of RORγt mRNA in patients the observation group was significantly higher than that of the control group.The levels of NK-T and Foxp3 mRNA were significantly lower than those in the control group,the difference was statistically significant(P<0.05);Compared with the stable period,the level of the RORγt mRNA of patients in the advanced stage is high;The levels of NK-T and Foxp3 mRNA were lower,and the difference was statistically significant(P<0.05);Among patients with vitiligo of different disease types,there was a statistically significant difference between the levels of NK-T,RORγt mRNA and Foxp3 mRNA(P<0.05);Between different lesion areas,there was a statistically significant difference between the levels of NK-T,RORγt mRNA and Foxp3 mRNA(P<0.05).There was a positive correlation between RORγt mRNA and lesion area(rs=0.509,P<0.05).NK-T and Foxp3 mRNA were negatively correlated with lesion area(rs=-0.348,rs=-0.307,P<0.05).【Conclusion】The abnormal levels of NK-T,RORγt mRNA and Foxp3 mRNA in peripheral blood are closely related to the onset and development of vitiligo,and the skin lesion area of vitiligo is positively correlated with RORγt mRNA,and negatively correlated with NK-T and Fox

关 键 词：白癜风 自然杀伤T细胞 转录因子/血液 叉头转录因子类/血液 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]