PKC-SWI6 signaling regulates asexual development,cell wall integrity,stress response,and lifestyle transition in the nematode-trapping fungus Arthrobotrys oligospora  

作　　者：Meihua Xie Ni Ma Na Bai Le Yang Xuewei Yang Ke-Qin Zhang Jinkui Yang 

机构地区：[1]State Key Laboratory for Conservation and Uilization of Bio-Resources,Key Laboratory for Microbial Resources of the Ministry of Education,School of Life Sciences,Yumman University,Kunming 650091,China [2]School of Resource,Emvironment and Chemistry,Chuciong Normal University,Chnuciong 675000,China [3]Yunnan Center for Disease Control and Prevention,Kunming 650022,China

出　　处：《Science China(Life Sciences)》2022年第12期2455-2471,共17页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(31960556,U1402265,32160665);the Applied Basic Research Foundation of Yunnan Province(202001BB050004);Postdoctoral Science Foundation of Yunnan Province。

摘　　要：Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the host.Herein,we characterized three components of the cell wall integrity-controlling pathway,namely protein kinase C(Ao PKC),SLT2-MAPK(Ao SLT2),and SWI6(Ao SWI6),in a representative nematode-trapping fungus Arthrobotrys oligospora,using gene disruption and multi-omics approaches.The phenotypic traits(such as mycelia development,conidiation,stress response,and trap morphogenesis) and metabolic profiles of ΔAopkc and ΔAoswi6 mutants were similar but differed from those of the ΔAoslt2 mutants.Transcriptomic analysis indicated that the genes differentially expressed in the absence of Aoswi6 were involved in DNA replication,repair,and recombination during trap formation.Moreover,the yeast two-hybrid assay showed that Ao PKC interacted with Ao SWI6,suggesting that in A.oligospora,PKC can directly regulate SWI6,bypassing the SLT2signaling cascade.Conclusively,our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.

关 键 词：Arthrobotrys oligospora CONIDIATION stress response trap formation differential expression analysis metabolic profile PATHOGENICITY 

分 类 号：Q935[生物学—微生物学]