Building the Chordata Olfactory Receptor Database using more than 400,000 receptors annotated by Genome2OR  

作　　者：Wei Han Yiran Wu Liting Zeng Suwen Zhao 

机构地区：[1]iHuman Institute,ShanghaiTech University,Shanghai 201210,China [2]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Science China(Life Sciences)》2022年第12期2539-2551,共13页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32122024,31971178);the National Key Research and Development Programs of China(2018YFA0507000);ShanghaiTech University。

摘　　要：Olfactory receptors are poorly annotated for most genome-sequenced chordates.To address this deficiency,we developed a nhmmer-based olfactory receptor annotation tool Genome2OR(https://github.com/To Hanwei/Genome2OR.git),and used it to process 1,695 sequenced chordate genomes in the NCBI Assembly database as of January,2021.In total,765,248 olfactory receptor genes were annotated,with 404,426 functional genes and 360,822 pseudogenes,which represents a four-fold increase in the number of annotated olfactory receptors.Based on the annotation data,we built a database called Chordata Olfactory Receptor Database(CORD,https://cord.ihuman.shanghaitech.edu.cn)for archiving,analysing and disseminating the data.Beyond the primary data,we offer derivative information,including pictures of species,cross references to public databases,structural models,sequence similarity networks and sequence profiles in the CORD.Furthermore,we did brief analyses on these receptors,including building a huge protein sequence similarity network covering all receptors in the database,and clustering them into 20 communities,classifying the 20 communities into three categories based on their presences/absences in ray-finned fish and/or lobe-finned fish.We infer that olfactory receptors should have unique activation and desensitization mechanisms by analysing their sequences and structural models.We believe the CORD can benefit the researchers and the general public who are interested in olfaction.

关 键 词：G protein-coupled receptors olfactory receptors Genome2OR CORD 

分 类 号：Q811.4[生物学—生物工程]