In vitro Antibacterial Activity of Palmitoleic Acid Isolated from Filamentous Microalga Tribonema minus Against Fish Pathogen Streptococcus agalactiae  

作　　者：WANG Feifei GUO Yuhao CAO Yan ZHANG Chengwu 

机构地区：[1]School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China [2]School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China [3]Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering,Ministry of Education,Guangzhou 510632,China [4]Department of Ecology and Research Center for Hydrobiology,Jinan University,Guangzhou 510632,China

出　　处：《Journal of Ocean University of China》2022年第6期1615-1621,共7页中国海洋大学学报（英文版）

基　　金：supported by the Jiangsu Provincial Natural Science Foundation(No.BK20200734);the Hubei Provincial Natural Science Foundation(No.2021CFB224);the Engineering Research Center for Tropical and Subtropical Aquatic Ecological Engineering,Ministry of Education,Jinan University,P.R.China(No.2021A0401);the Research and Innovation Initiatives of Wuhan Polytechnic University(WHPU)(No.2021Y06);the Hubei Key Laboratory for Processing and Transformation of Agricultural Products(WHPU,China)(No.2020HBSQGDKFB17);the Sinopec Joint Program of China Petroleum and Chemical Corporation(No.ST18005-2).

摘　　要：Filamentous microalgae from genus Tribonema are promising sustainable sources of omega-7 palmitoleic acid,but their ability to accumulate this compound varies among species and depends on the initial nitrogen concentration(INC)supply.In this study,the palmitoleic acid accumulation capacities of five Tribonema species were examined under three different INCs to select the alga species with the highest production.Results showed that a high INC was associated with increased palmitoleic acid accumulation but led to decreased biomass concentration in all tested species.In particular,T.minus grown at 18 mmol L^(−1)INC had the highest palmitoleic acid content(20.72%of dry weight)and productivity(90.88 mg L^(−1)d−1).The combination of alkali metal freezing precipitation(AMFP)and urea complexation successfully isolated and enriched palmitoleic acid from T.minus and obtained a purity of 80.11%and a yield of 7.39 g(100 g)^(−1) of algal powder.The compound was identified as(9Z)-hexadecenoic acid(C16:1ω-7).Antibacterial activity evaluation for the highly concentrated palmitoleic acid(10 mg mL^(−1))against Streptococcus agalactiae revealed the formation of a 12.10 mm-diameter inhibition zone and the minimum inhibitory concentration of 31.25μg mL^(−1),indicating that palmitoleic acid is an effective antibacterial agent.This study is the first to report that palmitoleic acid derived from T.minus can antagonize S.agalactiae,which further broadens the potential application of Tribonema biomass in green aquaculture.

关 键 词：Tribonema palmitoleic acid ENRICHMENT Streptococcus agalactiae antibacterial activity 

分 类 号：S948[农业科学—水产养殖]