Complete Mitochondrial Genome Analysis of Daphniopsis tibetana(Branchiopoda:Diplostraca):New Insights into the Taxonomy of the Genus and Its Phylogenetic Implications for Branchiopoda  

作　　者：LIU Bingjian PENG Ying LIU Yifan LI Jiasheng ZHANG Kun CHEN Jian GONG Li LIU Liqin LÜZhenming ZHANG Chi 

机构地区：[1]National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization,Zhejiang Ocean University,Zhoushan 316022,China [2]Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850002,China [3]Key Laboratory of Tropical Marine Bio-Resources and Ecology,Chinese Academy of Sciences,Guangzhou 510301,China

出　　处：《Journal of Ocean University of China》2022年第6期1622-1632,共11页中国海洋大学学报（英文版）

基　　金：supported by the Zhejiang Provincial Natural Science Foundation of China(Nos.LY22D060001,LY20C190008);the National Natural Science Foundation of China(Nos.41806156,31702321);the Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment(FEEL-2021-8);the Open Foundation from Key Laboratory of Tropical Marine Bio-resources and Ecology,Chinese Academy of Sciences(No.LMB20201005);the Science and Technology Project of Zhoushan(No.2020 C21016),the Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang(Nos.20200201,20200202);the Starting Research Fund from the Zhejiang Ocean University.

摘　　要：Daphniopsis tibetana is widely distributed in Xinjiang,Qinghai,Tibet of China,as well as in Russia and India,which is the dominant zooplankton in many high-altitude(4000 m)salt lakes.D.tibetana can adapt to saline waters,whereas the other species of the superorder Cladocera can only inhabit in freshwater.However,the phylogenetic status of D.tibetana in Branchiopoda remains unclear primarily because of limited mitogenome.In this study,complete mitochondrial genome sequences of D.tibetana were sequenced and annotated for the first time to obtain a comprehensive understanding of its phylogenetic status.The complete mitogenome of D.tibetana is 16196 bp in length.It contains 37 genes,including two ribosomal RNAs(12S and 16S rRNAs)genes,22 transfer RNA(tRNA)genes,13 protein-coding genes,and one non-coding region.The overall base composition is 29.6%A,33.2%T,19.0%G,and 18.2%C with a high AT bias(62.8%).Except for trnS1(GCT),most tRNAs have a typical cloverleaf secondary structure.Phylogenetic analysis based on maximum likelihood and Bayesian inference generates identical phylogenetic topology and shows the phylogenetic status of D.tibetana,which reconfirm the distinction between the genera Daphniopsis and Daphnia.Meanwhile,the class Branchiopoda is clustered into three clades(Anostraca,Notostraca,and Diplostraca)with high support values.These results provide not only a comprehensive understanding of the characteristics of D.tibetana mitogenome and its phylogenetic position in Diplostraca,but also information for future research on the phylogeny of Branchiopoda.

关 键 词：Daphniopsis tibetana MITOGENOME phylogenetic analysis BRANCHIOPODA Diplostraca 

分 类 号：S917.4[农业科学—水产科学]