木姜叶柯黄酮提取方法的研究  

A Study on the Extraction Methods of Lithocarpus Litseifolius Flavonids

作　　者：张俭 刘俊华[1,2] 李胜华 伍贤进 王依清[3,4] ZHANG Jian;LIU Jun-hua;LI Sheng-hua;WU Xian-jin;WANG Yi-qing(College of Biological and Food Engineering,Huaihua University,Huaihua,Hunan 418008;Key Laboratory of Rresearch and Utilization of Ethnomedicinal Plant Resources of Hunan Province,Huaihua,Hunan 418008;Hunan Yao Tea Engineering Research Center,Xupu,Hunan 419300;Hunan Aokang Biotechnology Co.,Ltd.,Xupu,Hunan 419300)

机构地区：[1]怀化学院生物与食品工程学院,湖南怀化418008 [2]民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]湖南省瑶茶工程技术研究中心,湖南溆浦419300 [4]湖南翱康生物科技有限公司,湖南溆浦419300

出　　处：《怀化学院学报》2022年第5期1-6,共6页Journal of Huaihua University

基　　金：湖南省科技创新计划项目“雪峰山道地中药材产业大学生创新创业人才培养项目”(2021RC1015);湖南省扶贫重点项目“黄精品种选育与示范推广”(18A455)。

摘　　要：分别采用70%乙醇回流提取、索氏提取和超声波辅助提取等3种方法对5份木姜叶柯材料的黄酮进行提取,利用高效液相色谱法(High Performance Liquid Chromatography,HPLC)分析所提黄酮成分.结果显示,回流提取法所得总黄酮的含量最高.高效液相色谱分析表明,回流提取法所得木姜叶柯总黄酮的含量均高于索氏提取的总黄酮的含量.乙醇回流提取木姜叶柯总黄酮较完全,优于索氏提取和超声波辅助提取.The flavonoids from 5 samples of Lithocarpus litseifolius were extracted by 70% ethanol reflux extraction,Soxhlet extraction and ultrasound-assisted extraction. The components of the extracted flavonoids were analyzed by HPLC.The content of total flavonoids obtained by the ethanol reflux extraction method was the highest. The analysis of five materials by HPLC showed that the total area of the flavonoids peaks in Lithocarpus litseifolius obtained by the reflux extraction method was higher than the total area of the peaks extracted by Soxhlet. The total flavonoids obtained by 70%ethanol reflux extraction is more complete and better than Soxhlet extraction and ultrasound-assisted extraction.

关 键 词：木姜叶柯 提取方法 黄酮类化合物 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]