日本医蛭进食前后唾液腺的形态学变化  

Morphological Changes of Salivary Glands in Hirudo nipponica before and after Feeding

作　　者：鲍亮 王泽冲 蔡美香 邢月婷 罗媛媛 BAO Liang;WANG Zechong;CAI Meixiang;XING Yueting;LUO Yuanyuan(College of Life Sciences,China Jiliang University,Hangzhou 310018,China;College of Medicine,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]中国计量大学生命科学学院,杭州310018 [2]浙江大学医学院,杭州310058

出　　处：《中国细胞生物学学报》2022年第7期1258-1266,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31440030)资助的课题。

摘　　要：为研究日本医蛭(Hirudo nipponica)进食前后唾液腺的形态和超微结构特征,该文通过光学显微镜和电子显微镜对日本医蛭唾液腺进行观察。基于光学显微镜成像,观察到日本医蛭咽部具有三角形肌肉颚,唾液腺呈葡萄状依附在颚上,腺体呈乳白色且对称分布在颚周围。HE染色结果表明,唾液腺细胞是由卵球形的体细胞和细长的导管组成的,且细胞核位于细胞底部或边缘。未进食的日本医蛭唾液腺细胞染色浅,细胞质饱满;进食后的日本医蛭唾液腺细胞染色深,细胞质结构疏松。基于透射电子显微镜成像,观察到唾液腺细胞中存在球形分泌颗粒,且分泌颗粒内部存在高电子密度的致密成分。未进食的日本医蛭唾液腺细胞结构紧致,分泌颗粒相互挤压,且大量分泌颗粒内含有致密成分;进食后的日本医蛭唾液腺细胞结构疏松,分泌颗粒之间存在空隙,且分泌颗粒内致密成分消失。基于扫描电子显微镜成像,观察到唾液腺细胞呈葡萄状排列。未进食的日本医蛭唾液腺细胞表面光滑,细胞圆润;进食后的日本医蛭唾液腺细胞表面存在大量颗粒物质,细胞凹陷。以上结果表明,日本医蛭进食后,唾液腺细胞分泌出的多种分泌蛋白是分泌颗粒中的致密成分,而大部分分泌颗粒还存在于唾液腺细胞内。To study the morphological and ultrastructural characteristics of salivary glands of Hirudo nipponica before and after feeding,the salivary glands of H.nipponica were observed by light microscope and electron microscope.Based on optical microscope imaging,it was observed that the pharynx of H.nipponica had a triangular muscle jaw,the salivary glands were attached to the jaw in a grape-like shape,and the glands were milky white and symmetrically distributed around the jaw.Results of HE staining showed that salivary gland cells were composed of ovoid somatic cells and elongated ducts,and the nuclei were located at the bottom or edge of the cells.The salivary gland cells of the unfed H.nipponica were lightly stained and the cytoplasm was plump;the salivary gland cells of the fed H.nipponica had dark staining and loose cytoplasm.Based on transmission electron microscopy imaging,spherical secretory granules were observed in salivary gland cells,and there was dense matter with high electron density inside the secretory granules.The salivary gland cells of the unfed H.nipponica were compact,the secretory granules squeezed each other,and a large number of secretory granules contained dense matter.However,the salivary gland cells of H.nipponica after feeding were loose in space,and there were gaps between secretory granules.Moreover,the dense matter in secretory granules disappeared.Based on scanning electron microscopy imaging,salivary gland cells were observed in a grape-like arrangement.The salivary gland cells of the unfed H.nipponica had smooth surface and rounded cells;the surface of the salivary gland cells of the fed H.nipponica had many extracellular particulates and the cells were pitted.The above results showed that various secretory proteins secreted by salivary gland cells were dense matter in the secretory granules after H.nipponica were feeding,and most of the secretory granules were still in the salivary gland cells.

关 键 词：日本医蛭 唾液腺细胞 超微结构 分泌颗粒 致密成分 

分 类 号：R282.74[医药卫生—中药学]