不同定量方法在婴儿配方奶粉中维生素B12测量中的不确定度评估  

Uncertainty Evaluation of Different Quantitative Calibration Methods for the Determination of Vitamin B12 in Infant Formula

作　　者：王许欣 李晓敏[1] 张庆合[1] WANG Xuxin;LI Xiaomin;ZHANG Qinghe(National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院,北京100029

出　　处：《计量科学与技术》2022年第9期23-27,46,共6页Metrology Science and Technology

基　　金：国家重点研发计划课题(2019YFC1604803)。

摘　　要：本文以婴儿配方奶粉中的维生素B12测量为例,开展了标准加入法、标准加入同位素稀释质谱法、单点同位素稀释质谱法和校准曲线同位素稀释质谱法四种定量方法的不确定度评估。基于ISO-GUM指南等不确定度评估方法对四种定量方法进行不确定度量化评估,发现方法重复性和曲线拟合引入的不确定度为主要不确定度来源,且标准加入法评估得到的不确定度明显大于同位素内标法评估得到的不确定度。通过不同定量方法的比较研究,可以更有效地选择定量方式,提高测量精度。In this study,take measurement of the vitamin B12 in infant formula as an example,measurement uncertainty was evaluated through four calibration methods,including standard addition method,standard addition-isotope dilution mass spectrometry method,single point isotope dilution mass spectrometry method,and standard calibration curve isotope dilution mass spectrometry method.The uncertainty evaluation of the four quantitative methods was assessed based on the ISO-GUM guidelines and other uncertainty assessment methods.The uncertainties introduced by the method of repeatability and curve fitting were the primary sources of uncertainty.Furthermore,the uncertainties obtained by the standard addition method were significantly more significant than those obtained by the isotope internal standard method.The comparative study of different quantification methods can lead to a more effective selection of quantification methods and improved measurement accuracy.

关 键 词：不确定度评估 同位素内标校准法 标准加入法 维生素B12 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程]