鲍曼不动杆菌耐药性与外排泵基因、外膜孔蛋白基因的相关性研究  

Study on correlation between drug resistance of Acinetobacter baumannii with efflux pump gene and outer membrane porin gene

作　　者：陈荣忠 杨子依 向蓉 CHEN Rongzhong;YANG Ziyi;XIANG Rong(Department of Clinical Laboratory,Affiliated Xiaolan Hospital,Southern Medical University,Zhongshan,Guangdong 528415,China;College of Medical Laboratory,Shaoyang University,Shaoyang,Hunan 422000,China)

机构地区：[1]南方医科大学附属小榄医院检验科,广东中山528415 [2]邵阳学院医学检验学院,湖南邵阳422000

出　　处：《国际检验医学杂志》2022年第24期2969-2972,2976,共5页International Journal of Laboratory Medicine

基　　金：广东省中山市医学科研项目(2021J327)。

摘　　要：目的探讨鲍曼不动杆菌的分子流行病学特征,检测其外排泵基因及外膜孔蛋白基因的携带情况,进一步研究外排泵及外膜孔蛋白与菌株耐药性的关系。方法收集广东省中山市西北部区域的鲍曼不动杆菌临床分离株,采用PCR技术扩增鲍曼不动杆菌的外排泵基因(ade B、ade R、ade S、ade J、abe M)以及外膜孔蛋白carO基因,同时对其进行抗菌药物耐药性检测。结果耐碳青霉烯类抗菌药物的鲍曼不动杆菌(CRABA)耐药性明显高于对碳青霉烯类抗菌药物敏感的鲍曼不动杆菌(CSABA),差异有统计学意义(P<0.05),其中有4株CRABA菌株对替加环素耐药。CRABA菌株中ade B、ade R、ade S以及ade J的检出率明显高于CSABA菌株,差异均具有统计学意义(P<0.05);abe M基因在CRABA与CSABA菌株中的检出率差异无统计学意义(P>0.05)。外膜孔蛋白carO基因在CRABA菌株中的检出率高于CSABA菌株,差异有统计学意义(P<0.05)。结论外膜孔蛋白以及外排泵系统是该地区CRABA的重要耐药机制之一,其中abe M基因可能与CRABA菌株耐药性无关。Objective To investigate the molecular epidemiological characteristics of Acinetobacter baumannii,to detect the carrying status of efflux pump gene and outer membrane porin gene,and to further study the relationship between the efflux pump and the outer membrane porin with the strain drug resistance.Methods The clinical isolated strains of Acinetobacter baumannii were collected from the northwest oregion of Zhongshan City,Guangdong Province.The efflux pump genes(ade B,ade R,ade S,ade J,abe M)and outer membrane porin carO gene of Acinetobacter baumannii were amplified by PCR technology,meanwhile their antimicrobial resistance was detected.Results The drug resistance of carbapenem-resistant Acinetobacter baumannii(CRABA)was significantly higher than that of carbapenem-sensitive Acinetobacter baumannii(CSABA),the difference was statistically significant(P<0.05).Among them,4 CRABA strains were resistant to tigecycline.The detection rates of ade B,ade R,ade S and ade J in CRABA strains were significantly higher than those in CSABA strains,and the difference was statistically significant(P<0.05).There was no statistical difference in the detection rates of Abe M gene between CRABA and CSABA strain(P>0.05).The detection rate of outer membrane porin carO gene in CRABA strains was higher than that in CSABA strains,and the difference was statistically significant(P<0.05).Conclusion The outer membrane porin and efflux pump system are one of the important drug resistance mechanisms of CRABA in this region,in which the abe M gene may not be related to the drug resistance of CRABA strains.

关 键 词：外排泵基因 外膜蛋白 碳青霉烯酶 鲍曼不动杆菌 

分 类 号：R446.5[医药卫生—诊断学]