葛根素对糖尿病性骨质疏松小鼠骨密度及氧化应激的影响  

Effect of Puerarin on Bone Mineral Density and Oxidative Stress in Mice with Diabetic Osteoporosis

作　　者：吕立桃 范卫闯 白登彦 马富海[3] LYU Litao;FANWeichuang;BAI Dengyan;MA Fuhai

机构地区：[1]开封市人民医院骨一科,河南开封475002 [2]甘肃省第二人民医院骨科,甘肃兰州730000 [3]甘肃省中医院骨科,甘肃兰州730000

出　　处：《新中医》2022年第21期7-12,共6页New Chinese Medicine

基　　金：甘肃省自然科学基金项目(21CX1RA176);甘肃省中医药管理局课题(GZK-2016-53)。

摘　　要：目的:观察葛根素对糖尿病性骨质疏松(DOP)小鼠骨密度及氧化应激的影响。方法:将45只KKAy小鼠给予高糖高脂饲料饮食和链脲佐菌素腹腔注射构建DOP模型,造模成功后随机分为模型组、二甲双胍组及葛根素组各15只,另取15只C57BL/6J小鼠作为对照组。治疗8周后,观察各组小鼠骨密度(BMD)、糖代谢指标[空腹血糖(FBG)、糖化血红蛋白(HbA1c)、空腹胰岛素(FINS)]、丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、8-羟基脱氧鸟苷(8-OHDG)、骨桥蛋白(OPN)及转化生长因子-β(TGF-β)水平;苏木精-伊红(HE)染色观察各组小鼠胰腺组织病理学改变;实时荧光定量聚合酶链反应法(RT-PCR)及蛋白免疫印迹法分别检测各组小鼠胰腺组织骨形态发生蛋白-6(BMP-6)和血管内皮生长因子(VEGF)mRNA及蛋白表达情况。结果:与对照组比较,模型组小鼠BMD、FINS、CAT和SOD明显降低(P<0.05),FBG、HbA1c、MDA、8-OHDG、OPN、TGF-β及胰腺组织BMP-6和VEGF mRNA及蛋白表达水平明显升高(P<0.05);模型组小鼠胰腺组织紊乱,细胞间隙增宽,可见大量中性粒细胞,细胞核受损。与模型组比较,二甲双胍组及葛根素组小鼠BMD、FINS、CAT和SOD水平明显上升(P<0.05),FBG、HbA1c、MDA、8-OHDG、OPN、TGF-β及胰腺组织中BMP-6和VEGF mRNA及蛋白表达水平明显降低(P<0.05);二甲双胍组及葛根素组干预后,小鼠胰腺组织损伤程度明显改善。结论:葛根素能够调节DOP小鼠胰腺组织BMP-6、VEGF mRNA及蛋白的表达,改善BMD,减少氧化应激损伤。Objective:To observe the effect of puerarin on bone mineral density and oxidative stress in mice with diabetic osteoporosis(DOP).Methods:A total of 45 KKAy mice were given high-glucose/high-fat diet and intraperitoneal injection with streptozotocin to establish a DOP model.After successful modeling,the mice were randomly divided into the model group,the metformin group and the puerarin group,with 15 mice in each group.Another 15 C57BL/6J mice were taken as the control group.After eight-week treatment,the levels of bone mineral density(BMD),glucose metabolism indexes including fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c)and fasting insulin(FINS),malondialdehyde(MDA),catalase(CAT),superoxide dismutase(SOD),8-hydroxydeoxyguanosine(8-OHDG),osteopontin(OPN)and transforming growth factor-β(TGF-β)were observed;hematoxylin-eosin staining(HE)was used to observe the pathological changes in pancreatic tissue in mice;real-time reverse transcriptionpolymerase chain reaction(RT-PCR)and Western Blot were used to respectively detect the mRNA and protein expressions of bone morphogenetic protein-6(BMP-6)and vascular endothelial growth factor(VEGF).Results:Compared with the control group,the levels of BMD,FINS,CAT and SOD in the model group were significantly decreased(P<0.05),and the levels of FBG,HbA1c,MDA,8-OHDG,OPN,TGF-βand the mRNA and protein expressions of BMP-6 and VEGF in pancreatic tissue were significantly increased(P<0.05);in the model group,the pancreatic tissue was disordered,the intercellular space was widened,a large number of neutrophils were visible,and the nucleus was damaged.Compared with the model group,the levels of BMD,FINS,CAT and SOD in the metformin group and the puerarin group were significantly increased(P<0.05),and the levels of FBG,HbA1c,MDA,8-OHDG,OPN,TGF-βand the mRNA and protein expressions of BMP-6 and VEGF in pancreatic tissue were significantly decreased(P<0.05);after the intervention,the degree of pancreatic tissue injury in mice in the metformin group and the puerarin group wa

关 键 词：糖尿病性骨质疏松 葛根素 氧化应激 骨密度 血管内皮生长因子 骨形态发生蛋白-6 

分 类 号：R587.1[医药卫生—内分泌]