常用“蒂达”类藏药材四数獐牙菜的成分分析  

Component Analysis of Swertiatetraptera

作　　者：苏媛 刘安平 刘学良 李维业 韩天楚 杨增亮 王开祥 刘海青 Su Yuan;Liu Anping;Liu Xueliang;Li Weiye;Han Tianchu;Yang Zengliang;Wang Kaixiang;Liu Haiqing(Xining Center for Food and Drug Inspection&Testing,Xining 810007,China;Qinghai Center for Drug Evaluation and Verification,Xining 810007,China)

机构地区：[1]西宁市食品药品检验检测中心,西宁810007 [2]青海省药品审评核查中心,西宁810007

出　　处：《中国药事》2022年第11期1280-1285,共6页Chinese Pharmaceutical Affairs

基　　金：青海省基础研究项目(编号2020-ZJ747)。

摘　　要：目的:建立高效液相色谱法同时测定四数獐牙菜中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基[口山]酮含量的方法。方法:采用Waters SunFire C18柱(4.6 mm×250 mm,5µm);以0.05%磷酸溶液-甲醇为流动相进行梯度洗脱;柱温30℃,流速1.0 mL·min^(-1),检测波长254 nm。结果:獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基[口山]酮分别在0.0320~0.3202μg(r=0.9997)、0.3788~3.7881μg(r=0.9998)、0.0124~0.1237μg(r=0.9995)、0.0032~0.0321μg(r=0.9998)、0.0031~0.0308μg(r=0.9994)进样质量范围内呈良好的线性关系,加样回收率分别为98.69%、97.81%、92.21%、93.44%、92.77%,RSD(r=6)分别为0.76%、1.77%、1.98%、1.59%、1.79%。7批样品中獐牙菜苦苷、龙胆苦苷、当药苷、当药醇苷、1-羟基-3,4,5三甲氧基[口山]酮的测定结果分别为0.1924%~0.3740%、2.1426%~4.4514%、0.1122%~0.1973%、0.0286%~0.0339%及0.0043%~0.0938%。结论:该方法简便、高效、灵敏、准确、经济实用,具有良好的重复性和稳定性,对于四数獐牙菜的质量控制、综合利用提供了有效参考依据。Objective:To establish a HPLC method for the simultaneous determination of Swertiamarin,Gentiopicrin,Sweroside,Dangyao alcohol glycoside and 1-hydroxy-3,4,5 trimethoxydione in Swertiatetraptera.Methods:Waters Sunfire C18 column(4.6 mm×250 mm,5μm)was used;gradient elution was carried out with methanol-0.05%phosphoric acid solution as mobile phase;the column temperature was 30℃,the flow rate was 1.0 mL·min^(-1),and the detection wavelength was 254 nm.Results:Five components have shown good linear relationship within the injection quality ranges of 0.0320-0.3202μg(r=0.9997),0.3788-3.7881μg(r=0.9998),0.0124-0.1237μg(r=0.9995),0.0032-0.0321μg(r=0.9998)and 0.0031-0.0308μg(r=0.9994)respectively.The recovery rates were 98.69%,97.81%,92.21%,93.44%and 92.77%(n=6),and RSDs(r=6)were 0.76%,1.77%,1.98%,1.59%and 1.79%.The results of the determination of the five components in 7 batches of samples were 0.1924%-0.3740%,2.1426%-4.4514%,0.1122%-0.1973%,0.0286%-0.0339%and 0.0043%-0.0938%respectively.Conclusion:The method is simple,efficient,sensitive,accurate,economical and practical with good repeatability and stability.It provides an effective reference for the quality control and comprehensive utilization of Swertiatetraptera.

关 键 词：四数獐牙菜 高效液相色谱法 獐牙菜苦苷 龙胆苦苷 当药苷 当药醇苷 1-羟基-3 4 5三甲氧基[口山]酮 含量测定 

分 类 号：R917[医药卫生—药物分析学]