42℃热疗抑制U251细胞增殖并诱导凋亡  

Hyperthermia at 42℃Inhibited The Proliferation and Induced Apoptosis of U251 Cells

作　　者：吴聪英 周翠 张捷[2] WU Cong-Ying;ZHOU Cui;ZHANG Jie(Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 101121,China;Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京中医药大学东直门医院,北京101121 [2]北京大学第一医院,北京100034

出　　处：《生物化学与生物物理进展》2022年第11期2240-2246,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(81901155)资助项目。

摘　　要：目的艾灸是一种经典的中医治疗手段,集合了物理疗法(热疗)和化学疗法,目前已经证明对多种癌症有抑制作用,但是具体的细胞生物学机制尚不明确。本研究旨在研究热疗对星形胶质细胞癌细胞系U251的作用,来揭示艾灸通过热疗对癌细胞的作用。方法通过显微缩时摄影技术,记录了42℃热疗处理U251细胞系108 h中细胞形态的变化,并用细胞计数法检测了热疗3 d时全细胞数、活细胞数及活细胞比例的变化,来评价热疗对细胞增殖和死亡的影响。通过检测细胞凋亡标志物Annexin V和Caspase-3,来评价热疗对细胞凋亡的作用。结果热疗明显抑制细胞的增殖,细胞死亡明显增加。热疗处理后的U251细胞较对照组存在明显的核固缩,标志物Annexin V和PI明显升高,PI阳性细胞呈Annexin V阳性,提示热疗导致的细胞死亡以细胞凋亡为主,PI阳性的细胞为凋亡终末期,细胞膜通透性增加所致。同时大部分收缩变圆的细胞中,Caspase-3和Annexin V呈共同阳性,提示热疗可能通过Caspase途径介导细胞凋亡。结论本研究明确了热疗可以抑制星形胶质细胞癌U251细胞系的细胞增殖并诱导Caspase途径相关的凋亡。Objective Moxibustion is a classic traditional Chinese medicine treatment that combines physical therapy(hyperthermia)and chemical therapy.It has been shown to have an inhibitory effect on a variety of cancers,but the cellular biological mechanism is still unclear.This study aims to study the effect of hyperthermia on astrocytic cancer cell line U251,and to reveal the effect of moxibustion on cancer cells through hyperthermia.Methods The morphological changes of U251 cells treated with hyperthermia at 42℃for 108 h were recorded by microphotography.The changes of total cell number,live cell number and live cell proportion were detected by cell counting after 3 d of hyperthermia to evaluate the effects of hyperthermia on cell proliferation and death.The effect of hyperthermia on cell apoptosis was evaluated by detecting Annexin V and Caspase-3.Results Hyperthermia significantly inhibited cell proliferation and increased cell death.Compared with the control group,the U251 cells treated with hyperthermia had obvious nuclear pyknosis,and Annexin V and PI markers were significantly increased,indicating that the cell death caused by hyperthermia was mainly caused by Caspase related apoptosis.Conclusion This study demonstrated that hyperthermia can inhibit cell proliferation and induce Caspase related apoptosis in astrocytic cell line U251.

关 键 词：热疗 细胞凋亡 星形胶质细胞瘤 

分 类 号：R11[医药卫生—公共卫生与预防医学] Q2[生物学—细胞生物学]