外周血血清白细胞介素-6和白细胞介素-8联合检测诊断宫内感染的效果  

Effect of combined detection of interleukin-6 and interleukin-8 in peripheral serum in diagnosis of intrauterine infection

作　　者：陈海慧 蔡安利 项军淼 林琳[1] 赵丽芬 CHEN Hai-Hui;CAI An-Li;XIANG Jun-Miao(Department of Obstetrics and Gynecology,Ruian People's Hospital,Ruian,Zhejiang 325200,China)

机构地区：[1]瑞安市人民医院妇产科,浙江瑞安325200 [2]瑞安市人民医院检验科,浙江瑞安325200

出　　处：《中国妇幼保健》2022年第21期3902-3906,共5页Maternal and Child Health Care of China

基　　金：浙江省瑞安市科技创新项目(MS2018015)。

摘　　要：目的 检测孕妇外周血血清白细胞介素-6(IL-6)和白细胞介素-8(IL-8)表达水平,探讨二者联合检测对孕妇宫内感染的诊断价值和应用效果。方法 选取2017年6月-2019年6月在瑞安市人民医院住院分娩并发生胎膜早破的孕妇120例为观察组,其中经胎盘病理检查确诊患有绒毛膜羊膜炎的孕妇45例作为感染组,确诊未感染孕妇75例作为未感染组,另选取同期在该院门诊定期产检且足月分娩的健康孕妇110例作为对照组。采用酶联免疫吸附法(ELISA)检测所有研究对象血清和羊水标本中IL-6、IL-8水平;分析感染组患者血清IL-6、IL-8水平与临床病理参数的关系;ROC曲线分析血清IL-6、IL-8水平对宫内感染的诊断价值;比较感染组和未感染组患者年龄、感染程度及妊娠结局情况,并比较C-反应蛋白(CRP)、白细胞(WBC)、肿瘤坏死因子(TNF-α)、血清IL-6、IL-8及两者联合诊断对宫内感染的诊断价值。结果 感染组患者血清IL-6和IL-8水平[(529.77±132.49)pg/ml、(741.15±435.64)pg/ml]均高于对照组[(319.65±118.09)pg/ml、(534.15±111.43)pg/ml],差异均有统计学意义(t=13.628、13.231,均P<0.05);感染组患者血清和羊水IL-6、IL-8水平[血清:(529.77±132.49)pg/ml、(741.15±435.64)pg/ml,羊水:(538.74±134.70)pg/ml、(803.64±120.55)pg/ml]均高于未感染组[血清:(357.36±124.89)pg/ml、(558.94±136.85)pg/ml,羊水:(338.11±133.74)pg/ml、(596.78±114.82)pg/ml],差异均有统计学意义(血清t=10.494、10.930,羊水t=6.400、7.494,均P<0.05)。血清IL-6、IL-8水平与患者的感染程度、妊娠结局有关(均P<0.05)。血清IL-6对宫内感染诊断的曲线下面积(AUC)为0.790,灵敏度为75.0%,特异度为76.2%;IL-8对宫内感染诊断的AUC为0.800,灵敏度为63.9%,特异度为86.9%;两者联合检测的AUC为0.874,灵敏度为83.3%,特异度为81.0%。感染组患者血清CRP、WBC及TNF-α水平[(14.03±3.27)mg/L、(13.75±4.63)×10^(9)/L及(682.18±284.14)ng/ml]水平均显著高于未感Objective To measure the expression levels of interleukin-6(IL-6) and interleukin-8(IL-8) in the peripheral blood of pregnant women, and to explore the diagnostic value and application effect of the combined detection of interleukin-6(IL-6) and interleukin-8(IL-8) in intrauterine infection of pregnant women.Methods From June 2017 to June 2019, 120 pregnant women with premature rupture of membranes delivered in Ruian People’s Hospital were selected as the observation group, among them, 45 pregnant women with chorioamnionitis confirmed by placental pathology were taken as the infected group, and 75 pregnant women without chorioamnionitis confirmed by placental pathology were taken as the uninfected group, in addition, 110 healthy pregnant women with regular antenatal examination and full-term delivery in the outpatient department of the hospital were selected as the control group.The levels of IL-6 and IL-8 in serum and amniotic fluid of all subjects were detected by ELISA;the relationship between serum IL-6, IL-8 levels and clinicopathological parameters was analyzed;ROC curve was used to analyze the diagnostic value of serum IL-6 and IL-8 levels for intrauterine infection;the age, infection degree and pregnancy outcome of patients in the infected group and the uninfected group were compared, the diagnostic value of C-reactive protein(CRP), white blood cell(WBC), tumor necrosis factor(TNF-α), serum IL-6, IL-8 and their combination for intrauterine infection were compared.Results The levels of serum IL-6 and IL-8 in the infected group [(529.77±132.49) pg/ml,(741.15±435.64) pg/ml] were higher than those in the control group [(319.65±118.09) pg/ml,(534.15±111.43) pg/ml], the difference was statistically obvious(t=13.628, 13.231, all P<0.05);the levels of IL-6 and IL-8 in serum and amniotic fluid in the infected group [serum:(529.77±132.49) pg/ml,(741.15±435.64) pg/ml, amniotic fluid:(538.74±134.70) pg/ml,(803.64±120.55) pg/ml] were higher than those in the uninfected group [serum:(357.36±124.89) pg/ml,(55

关 键 词：宫内感染 白细胞介素-6 白细胞介素-8 联合检测 

分 类 号：R711[医药卫生—妇产科学]