2017—2020年无锡市人腺病毒流行特征及B3亚型Hexon基因变异分析  

Epidemiological characteristics and B3 hexon gene variation of human adenovirus in Wuxi from 2017 to 2020

作　　者：马广源 肖勇[1] 鲍静 管红霞 周琪 於淳安 Ma Guangyuan;Xiao Yong;Bao Jing;Guan Hongxia;Zhou Qi;Yu Chun'an(The Affiliated Wuxi Center for Disease Control and Prevention of Nanjing Medical University,Wuxi 214023,China)

机构地区：[1]南京医科大学附属无锡疾病预防控制中心,无锡214023

出　　处：《中华实验和临床病毒学杂志》2022年第5期569-574,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：无锡市卫健委中青年拔尖人才资助计划(HB2020094);无锡市科技局项目(N20192042)。

摘　　要：目的了解2017—2020年无锡市人腺病毒(human adenovirus,HAdV)的基因分型、流行特征及主要流行株的基因变异情况。方法使用实时荧光PCR技术检测2017—2020年哨点医院送检急性呼吸道感染标本,荧光PCR检测阳性标本经细胞分离培养,Hexon基因测序、分析,确定基因型别及变异。结果2017—2020年无锡市HAdV检出情况略有不同,总体在5~7月和11~次年1月份出现2个流行峰。病例主要为幼儿(1~3岁),阳性标本占比为32.05%(75/234)。临床症状主要为发热占比为65.81%,其次是咳嗽占比为58.12%。检出HAdV有8个型别,分属3个亚属,HAdV-3已成为主要流行株,占比为47.18%(67/142),其余HAdV-713.38%(19/142),HAdV-557.75%(11/142),HAdV-19.15%(13/142),HAdV-29.15%(13/142),HAdV-55.63%(8/142),HAdV-62.11%(3/142),HAdV-45.63%(8/142)。本地流行株HAdV-3与9株国内和国际(KX384958.1-USA)参考株比较,HAdV-3在进化过程中623位、938位出现氨基酸的突变。结论2017—2020年无锡市HAdV在5~7月和11~次年1月出现2个流行峰,病例主要为1~3岁的幼儿,HAdV-3已逐渐成为优势流行株,在压力选择和进化过程中出现2个高频突变位点。Objective To understand the genotyping,epidemic characteristics,genetic variation and main epidemic strains of human adenovirus(HAdV)in Wuxi city from 2017 to 2020.Methods Real-time fluorescence PCR was used to detect the acute respiratory tract infection cases by testing the specimens submitted to sentinel hospitals from 2017 to 2020.The specimens of positive cases tested for nucleic acid were subjected to viral isolation via cell culture,Hexon gene sequencing and analysis were performed to determine genotype and variation.Results The detection of human adenovirus was slightly different,there were two epidemic peaks in May to July and November to January in Wuxi from 2017 to 2020.The cases were mainly infants(1-3 years old),and the positive samples accounted for 32.05%(75/234).The main clinical symptoms were fever(65.81%),followed by cough(58.12%).There were 8 types of human adenovirus,belonging to 3 subgenera.HAdV-3 has become the main epidemic strain,accounting for 47.18%(67/142),the rest HAdV-713.38%(19/142),HAdV-557.75%(11/142),HAdV-19.15%(13/142),HAdV-29.15%(13/142),HAdV-55.63%(8/142),HAdV-62.11%(3/142),HAdV-45.63%(8/142).Compared with 9 domestic reference strains and international(KX384958.1-USA)reference strains,the local epidemic strain HAdV-3 has amino acid mutations at positions 623 and 938 in the evolutionary process.Conclusions From 2017 to 2020,there are two epidemic peaks.The cases are mainly infants 1 to 3 years old.HAdV-3 has gradually become the dominant epidemic strain,and there are two high frequency mutation sites in the process of pressure selection and evolution.

关 键 词：人腺病毒 Hexon基因 变异 

分 类 号：R725.1[医药卫生—儿科]