2013—2019年徐州地区儿童流感样病例中腺病毒分子流行病学调查  

Molecular epidemiological analysis of adenovirus in children with influenza-like illness in Xuzhou from 2013 to 2019

作　　者：祝雯雯[1] 吴畏畏 高雪 许艳平 张文婷 杜阳光[1] 童晶[1] Zhu Wenwen;Wu Weiwei;Gao Xue;Xu Yanping;Zhang Wenting;Du Yangguang;Tong Jing(Laboratory Department,Xuzhou Center For Disease Control and Prevention,Xuzhou 221006,China)

机构地区：[1]徐州市疾病预防控制中心检验科,徐州221006

出　　处：《中华实验和临床病毒学杂志》2022年第5期575-578,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：江苏省预防医学课题(Y2018014)。

摘　　要：目的了解徐州地区儿童腺病毒感染的流行学特征。方法收集2013—2019年徐州市儿童医院流感样患儿咽拭子标本7257例,采用Real-time PCR方法检测腺病毒,阳性标本扩增其六邻体基因进行型别分析,对腺病毒55型采用Hep-2细胞分离培养并全基因测序分析。结果7257例患儿共检出227例腺病毒阳性,检出率为3.13%,感染主要集中在3~<6岁年龄组儿童,其中检出高峰为5月和6月份;型别分析结果显示,腺病毒感染主要为腺病毒3型和7型,不同年份流行优势型别在腺病毒3型和7型之间更替。腺病毒55型经细胞分离全基因测序,发现仅有少数碱基点突变,未发生集中区域的基因突变和重组。结论2013—2019年徐州地区儿童流感样病例中腺病毒以3和7型为主,感染人群以学龄前儿童为主,检出高峰为春夏季。Objective To understand the epidemiological characteristics of human adenovirus(HAdv)in Xuzhou area.Methods A total of 7257 swab samples from influenza-like illness children in Xuzhou children’s Hospital were collected during 2013-2019.Real-time PCR was used to detect adenovirus,and then the positive specimens were subjected to amplification for the hexon gene by polymerase chain reaction(PCR)for genotypic analysis.Adenovirus 55 was cultured by Hep-2 cells and analyzed by whole gene sequencing.Results A total of 227 strains of adenoviruses were detected out of 7257 samples,and the detection rate was 3.13%.Infections were mainly found in children aged 3 to 6,with a peak of detection in May and June.Typing data showed that the infection was mainly adenovirus type 3 and type 7,and the dominant type of adenovirus in different years alternated between type 3 and type 7.Complete gene sequencing showed only a few point mutations in genes of adenovirus type 55 we isolated,and no other gene mutation and recombination were found.Conclusions The main types of adenovirus were types 3 and 7 among children with inluenza-like illness in Xuzhou from 2013 to 2019,and the infected population was mainly preschool children,and the detection peak was in spring and summer.

关 键 词：腺病毒 儿童 型别检测 分子流行病学 

分 类 号：R725.1[医药卫生—儿科]