Tissue Culture and Rapid Propagation System of Roselle(Hibiscus sabdariffa L.)  

作　　者：Zifan GUI Fangjun LIAO Xiaoling YE Sumei HUANG Liqing WANG 

机构地区：[1]Shenzhen Park Management Center,Shenzhen 518000,China [2]Huizhou Engineering Vocational College,Huizhou 516023,China [3]Huizhou Science Research Institute for Agriculture,Huizhou 516023,China

出　　处：《Agricultural Biotechnology》2022年第6期74-77,共4页农业生物技术（英文版）

基　　金：Supported by Project of Bureau of Science and Technology of Huizhou Municipality(2020SD0409037).

摘　　要：[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle.

关 键 词：Hibiscus sabdariffa L. Tissue culture POLYPLOID Survival ratio 

分 类 号：S567.219[农业科学—中草药栽培]