安徽甘蓝菌核病菌的致病力及其对3种杀菌剂的敏感性  

Pathogenicity of Cabbage Sclerotinia sclerotiorum Isolatesin Anhui Province and Their Sensitivity to 3 Fungicides

作　　者：张华蕊 夏智杰 宋江华[2] 张立新[1] ZHANG Huarui;XIA Zhijie;SONG Jianghua;ZHANG Lixin(Anhui Province Key Laboratory of Integrated Pest Management on Crops,College of Plant Protection,Anhui Agricultural University,Hefei 230036,Anhui,China;College of Horticulture,Anhui Agricultural University,Hefei 230036,Anhui,China)

机构地区：[1]安徽农业大学植物保护学院,作物有害生物综合治理安徽省重点实验室,安徽合肥230036 [2]安徽农业大学园艺学院,安徽合肥230036

出　　处：《中国蔬菜》2022年第12期59-63,共5页China Vegetables

基　　金：国家自然科学基金项目(32272728)。

摘　　要：2020—2021年从安徽省甘蓝主产区的4个菌核病发生地块采集病株并分离、纯化菌株,通过菌丝圆片活体接种法测定各菌株对9个甘蓝品种的致病力;同时采用菌丝生长速率法测定代表性菌株对菌核净、多菌灵和啶酰菌胺的敏感性。结果表明:分离、纯化的24株甘蓝菌核病菌的致病力表现出明显差异,不同菌株对同一甘蓝品种的致病力不同,同一地区内的菌株致病力也存在明显差异,但菌株的致病性分化与地理来源无明显相关性;参试菌株对菌核净、多菌灵和啶酰菌胺的敏感性均较高,其中对多菌灵敏感性最高,EC_(50)值范围为0.0371~0.1042μg·mL^(-1),对菌核净和啶酰菌胺的EC_(50)值分别为0.0554~0.1377μg·mL^(-1)和0.0744~0.1870μg·mL^(-1)。This experiment collected infected plants from 4 Sclerotinia sclerotiorum infected plots in main cabbage production areas of Anhui Province in 2020-2021,and isolated and purified the bacterial isolates.The pathogenicity of each isolate of 9 cabbage varieties was tested by in vivo inoculation of mycelium disc.At same time,the sensitivities of representative isolates to dimethachlon,carbendazim and boscalid were determined by mycelial growth rate method.The results showed that the pathogenicity of 24 isolates of S.sclerotiorum showed significant differences.The pathogenicity of different isolates was different on the same cabbage variety.There were also obvious differences in pathogenicities of isolates from the same region.But,there was no significant correlation between pathogenicity differentiation of isolates and geographical origin.All tested isolates were higher sensitive to dimethachlon,carbendazim and boscolid.Therein,sensitivity to carbendazim was the highest,EC_(50) value ranging 0.0371-0.1042μg·mL^(-1).The EC_(50) value of dimethachlon and boscolid were 0.0554-0.1377μg·mL^(-1) and 0.0744-0.1870μg·mL^(-1),respectively.

关 键 词：甘蓝菌核病 致病力 杀菌剂 敏感性 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]