超声辅助提取冬荪菌托多糖的工艺优化  

Optimization of Ultrasound-assisted Extraction Technique of Polysaccharides from Volva of Phallus impudicus

作　　者：蒲晗旭 张露 李茂淋 陶烽 王静[1,2] 罗鹏 王国泽[1,2] PU Hanxu;ZHANG Lu;LI Maolin;TAO Feng;WANG Jing;LUO Peng;WANG Guoze(School of Public Health and Wellness/the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025,China;Guizhou Provincial Engineering Research Center of Food Nutrition and Health,Guiyang 550025,China;College of Food and Biological Engineering,Chengdu University,Chengdu 610106,China)

机构地区：[1]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵阳550025 [2]贵州省食品营养与健康工程研究中心,贵阳550025 [3]成都大学食品与生物工程学院,四川成都610106

出　　处：《中国果菜》2022年第12期41-47,共7页China Fruit & Vegetable

基　　金：贵州省科技厅科技计划项目(黔科合支撑[2022]一般146/[2021]一般134、黔科合基础ZK[2021]一般169);贵州省教育厅自然科学研究项目(黔教合KY字[2021]008/155);贵州医科大学药用植物功效利用国家重点实验室(FAMP202009K)。

摘　　要：本文采用超声辅助水提法提取冬荪菌托多糖,并通过Sevag手段脱蛋白,通过单因素试验和响应面法,以冬荪菌托多糖提取得率为衡量指标,优化提取工艺。结果表明,冬荪菌托多糖的最佳提取工艺条件为水浴温度92℃,超声功率535 W,超声时间32 min,液料比27∶1(mL/g)。此时多糖提取得率为7.442%,与模型预测理论值7.568%相近。本研究优化所得工艺可为提取冬荪菌托多糖提供参考。In this paper,ultrasonic-assisted water extraction was used to extract polysaccharides from Phallus impudicus and deproteinized by Sevag method.The extraction rate of polysaccharides from dictyophora Phallus impudicus was optimized by single factor test and response surface method.The results showed that the optimal extraction conditions were water bath temperature 92℃,ultrasonic power 535 W,ultrasonic time 32 min and liquid-solid ratio 27∶1(mL/g).At this time,the extraction rate of polysaccharide was 7.442%,which was close to the theoretical value of 7.568%predicted by the model.The optimized process can provide reference for the extraction of polysaccharides from Dictyophora.

关 键 词：冬荪菌托 多糖 超声提取 响应面法 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]