珠子参总皂苷上调miR-216a抑制人巨细胞病毒感染人胚肺成纤维细胞损伤  

Rhizome panacis majoris total saponin inhibits human cytomegalovirus infection of human embryonic lung fibroblasts by up-regulating miR-216a

作　　者：高山 庞婕 李占结 李晨希 尚庆毅[4] Gao Shan;Pang Jie;Li Zhanjie;Li Chenxi;Shang Qingyi(Department of Pharmacy,the First People's Hospital of Lianyungang,Lianyungang 222006,China;Department of Infection,Jiangsu Province Hospital,Nanjing 210036,China;School of Medical Technology,Xuzhou Medical University,Xuzhou 221004,China;Department of Laboratory Medicine,Ganyu District People's Hospital of Lianyungang City,Lianyungang 222199,China)

机构地区：[1]连云港第一人民医院药学部,江苏连云港222006 [2]江苏省人民医院感染科,南京210036 [3]徐州医科大学医学技术学院,江苏徐州221004 [4]连云港市赣榆区人民医院检验科,江苏连云港222199

出　　处：《中国临床解剖学杂志》2022年第6期689-695,共7页Chinese Journal of Clinical Anatomy

摘　　要：目的探讨珠子参总皂苷对人巨细胞病毒(HCMV)感染人胚肺成纤维细胞(MRC-5)炎症反应、凋亡的影响及其对miR-216a的调控作用。方法采用HCMV感染MRC-5细胞建立病毒感染模型,不同浓度(100μg/mL、200μg/mL、400μg/mL)的珠子参总皂苷处理细胞,分别将miR-NC、miR-216a mimics转染至HCMV感染的MRC-5细胞,分别将anti-miR-NC、anti-miR-216a转染至HCMV感染的MRC-5细胞后加入珠子参总皂苷处理细胞;采用ELISA法检测TNF-α、IL-6水平,流式细胞术检测细胞凋亡率,qRT-PCR法检测miR-216a表达量。结果珠子参总皂苷可降低HCMV感染的MRC-5细胞凋亡率及TNF-α、IL-6的水平(P<0.05),而miR-216a的表达水平升高(P<0.05);转染miR-216a mimics可降低凋亡率及TNF-α、IL-6的水平(P<0.05);转染anti-miR-216a可降低珠子参总皂苷对HCMV感染的MRC-5细胞炎症反应及凋亡的作用。结论珠子参总皂苷可通过上调miR-216a的表达而抑制炎症反应及细胞凋亡从而减轻HCMV感染的MRC-5细胞损伤。Objective To explore the effect of rhizome panacis majoris total saponin on the inflammatory response and apoptosis of human embryonic fibroblast(MRC-5)infected by human cytomegalovirus(HCMV)and its regulatory effect on miR-216a.Methods MRC-5 cells were infected with HCMV to establish a virus infection model.The cells were treated with panacis majoris rhizome total saponin at different concentrations(100,200,400μg/mL).The miR-NC and miR-216a mimics were transfected into HCMV-infected MRC-5 cells.After transfecting anti-miR-NC and anti-miR-216a into HCMV-infected MRC-5 cells,rhizome panacis majoris total saponin was added to the cells.ELISA was used to detect the levels of TNF-αand IL-6.Flow cytometry was used to detect the apoptosis rate.qRT-PCR method was used to detect the expression of miR-216a.Results Rhizome panacis majoris total saponin could reduce the apoptosis rate and the levels of TNF-αand IL-6 in MRC-5 cells infected with HCMV(P<0.05),while increase the expression level of miR-216a(P<0.05).Transfection of miR-216a mimics could reduce the rate of apoptosis and the levels of TNF-αand IL-6(P<0.05).Transfection of anti-miR-216a could reduce the effect of rhizome panacis majoris total saponin on the inflammation and apoptosis of MRC-5 cells infected with HCMV.Conclusions Rhizome panacis majoris total saponin can inhibit the inflammatory response and apoptosis by up-regulating the expression of miR-216a,thereby reducing the damage of MRC-5 cells infected by HCMV.

关 键 词：珠子参总皂苷 miR-216a 人巨细胞病毒 人胚肺成纤维细胞 凋亡 炎症 

分 类 号：R285[医药卫生—中药学]