重组基因Ⅶ型新城疫病毒疫苗候选株的构建  

Construction of candidate strains of recombinant Ⅶ Newcastle disease virus for vaccine

作　　者：周洁 孙慧敏 董嘉文 李林林 张俊勤 黄允真 汪招雄[2] 孙敏华 ZHOU Jie;SUN Huimin;DONG Jiawen;LI Linlin;ZHANG Junqin;HUANG Yunzhen;WANG Zhaoxiong;SUN Minhua(Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases,Ministry of Agriculture and Rural Affairs,Key Laboratory of Livestock Disease Prevention and Treatment of Guangdong,Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology,Guangzhou Guangdong 510640;Yangtze University College of Animal Science,Jingzhou Hubei 434025;Zhejiang Difference Biotechnology Co.,Ltd,Hangzhou Zhejiang 310052)

机构地区：[1]广东省农业科学院动物卫生研究所,农业农村部禽流感等家禽重大疾病防控重点实验室,广东省畜禽疫病防治研究重点实验室,岭南现代农业科学与技术广东省实验室茂名分中心,广东广州510640 [2]长江大学动物科学学院,湖北荆州434025 [3]浙江迪福润丝生物科技有限公司,浙江杭州310052

出　　处：《广东畜牧兽医科技》2022年第6期10-14,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：广州市科技计划项目(201904010379);广东省自然科学基金项目(2018A0303130003)。

摘　　要：新城疫是我国必须控制和净化的传染病之一,疫苗免疫是防控疾病的重要手段。为获得抗原性匹配的疫苗株,以新城疫LaSota疫苗株为骨架,替换当前流行的基因Ⅶ型毒株GM株的主要抗原性相关基因F(Fusion)和HN(Hemagglutinin-neuraminidase),拯救获得重组病毒rLa-mGM。生物学特性测定表明,重组毒株的MDT为160 h,ICPI为0,EID_(50)为10^(-8.4)/0.2 mL。将rLa-mGM株繁殖后以10^(8.0) EID_(50)制备灭活疫苗,与商品化LaSota疫苗各免疫10只25日龄SPF鸡,0.2 mL/只。免疫后14天,其血清平均HI效价为7.6log2,商品化LaSota疫苗免疫鸡的血清平均HI效价为7.4log2。结果表明重组病毒免疫原性良好,可作为疫苗候选株。Newcastle disease is an infectious disease that must be controlled and eradicated in China.In order to generate antigen-matched vaccine,the main antigenic-related fusion and hemagglutinin-neuraminidase genes of LaSota vaccine strain were replaced by those genes of GM strain belonging to genotypeⅦ,and the recombinant virus rLa-mGM was obtained by virus rescue.Biological characteristics of rLa-mGM showed that the MDT of the recombinant strain was 160 h,the ICPI was 0,and the EID_(50) was 10^(-8.4)/0.2 mL.The rLa-mGM strain was propagated to prepare an inactivated vaccine at 10^(8.0) EID_(50) and immunized 10 SPF chickens of 25-day-old with 0.2 ml/each.At 14 days after immunization,the average HI titer of serum collected from rLa-mGM inactivated vaccine and commercial LaSota vaccine was 7.6log2 and 7.4log2,respectively.These results indicates that the recombinant virus is immunogenic and can be used as a vaccine candidate strain.

关 键 词：新城疫 基因Ⅶ型 LaSota疫苗株 重组毒株 HI效价 疫苗候选株 

分 类 号：S852.65[农业科学—基础兽医学]