柔嫩艾美耳球虫ETYF株的生物学特性研究  

Research on Biological Characteristics of Eimeria tenella ETYF strains

作　　者：林栩慧 魏光伟 黄光宇 黄耿雄 戚南山 李娟 严专强 尹丽娟 余水兰 吕敏娜 胡俊菁 蔡海明 廖申权 孙铭飞 LIN Xuhui;WEI Guangwei;HUANG Guangyu;HUANG Gengxiong;QI Nanshan;LI Juan;YAN Zhuanqiang;YIN Lijuan;YU Shuilan;LV Minna;HU Junjing;CAI Haiming;LIAO Shenquan;SUN Mingfei(Yunfu Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Yunfu Guangdong 527400;Key Laboratory of Livestock Disease Prevention of Guangdong Province,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control,Ministry of Agriculture and Rural Affairs,Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;College of Animal Science and Technology Zhongkai University of Agriculture and Engineering,Guangzhou Guangdong 510225)

机构地区：[1]岭南现代农业科学与技术广东省实验室云浮分中心,广东云浮527400 [2]广东省农业科学院动物卫生研究所,广东省畜禽疫病防治研究重点实验室,农业农村部禽流感等家禽重大疾病防控重点实验室,广东广州510640 [3]仲恺农业工程学院,广东广州510225

出　　处：《广东畜牧兽医科技》2022年第6期15-20,39,共7页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省科技计划项目(2021B1212050021);云浮市科技计划项目(2021040202,2021020604,2021020607);广州市科技计划项目(202102080459);广东省现代农业产业技术体系创新团队建设专项(2022KJ119);广东省农业科学院协同创新中心项目(XTXM202202)。

摘　　要：利用单卵囊分离技术、动物传代、显微镜镜检、PCR扩增等方法,对分离自广东云浮鸡场的柔嫩艾美耳球虫(Eimeria tenella YuFu,ETYF株)进行研究,明确其卵囊指数、潜隐期、致病力、排卵囊规律等生物学特性。结果发现:该虫株卵囊平均大小为22.34μm×19.21μm,卵囊指数为1.16±0.17,最早潜隐期为144 h;不同感染剂量组排卵囊高峰期不一致,主要集中在162-180 h,感染3万个/只剂量组排卵囊总量最高,达1447万个/只;明确其最适卵囊接种剂量为1~3万个/只;感染5万个/只ETYF孢子化卵囊后出现死鸡,明确其最低致死剂量为5万个孢子化卵囊,且感染剂量越大,鸡肠道的病变越严重。本研究为后续药物筛选和疫苗研发提供数据支持,对防治鸡球虫病具有一定的研究意义。In this study,Eimeria tenella YuFu(ETYF strain)was isolated from a broiler farm in YuFu city,Guangdong Province. The methods of animal passage,microscopy,and PCR were used to identify Eimeria tenella. Coccidia ETYF species and its biological characteristics such as minimum prepatent period,oocyst excretion pattern,pathogenicity,and pathogenicity were monitored. The results showed that the minimum prepatent period could be continuously monitored 144 hours after the chickens were infected with ETYF strains of coccidia;Among them,the ET-3 group(30000 piece/bird dose)had the highest peak at 168~174hours after infection with ETYF,and the total number of oocyst excretion reached the largest during this period,reaching as high as about 14.47 million piece/dose. The optimal inoculation dose was 10000-30000 piece/bird dose;dead chickens appeared in ET-5(50,000 piece/bird dose)and ET-10(100,000 piece/bird dose),and the pathogenicity dose was 5.0×104piece/bird dose. This study provides data support for subsequent drug screening and vaccine development. which has certain significance for the control of chicken coccidiosis.

关 键 词：柔嫩艾美耳球虫 潜隐期 生物学特性 致病 

分 类 号：S852.723[农业科学—基础兽医学]