Momordica charantia fruit extract with antioxidant capacity improves the expression of tyrosine-phosphorylated proteins in epididymal fluid of chronic stress rats  

作　　者：Supatcharee Arun Therachon Kamollerd Nareelak Tangsrisakda Sudtida Bunsueb Arada Chaiyamoon Alexander Tsang-Hsien Wu Sitthichai Iamsaard 

机构地区：[1]Department of Anatomy,Faculty of Medicine,Khon Kaen University,Khon Kaen 40002,Thailand [2]Research Institute for Human High Performance and Health Promotion(HHP&HP),Khon Kaen University,Khon Kaen 40002,Thailand [3]The PhD Program for Translational Medicine,College of Medical Science and Technology,Taipei Medical University and Academia Sinica,Taipei 11031,Taiwan,China [4]Graduate Institute of Medical Sciences,National Defense Medical Center,Taipei 114,Taiwan,China

出　　处：《Journal of Integrative Medicine》2022年第6期534-542,共9页结合医学学报（英文版）

基　　金：financially supported by the young researcher development project of Khon Kaen University。

摘　　要：Objective:Although the protective effects of Momordica charantia L.(MC)extract on chemical-induced testicular damage have been studied,the preventive effects of MC extract on functional proteins in the epididymis under chronic stress have never been reported.This study investigated the protective effects of MC fruit extract on protein secretion,especially tyrosine-phosphorylated proteins,in the epididymis of rats exposed to chronic unpredictable stress(CUS).Methods:Total phenolic compounds(TPC),total flavonoid compounds(TFC)and antioxidant capacities of MC extract were measured.Adult male rats were divided into 4 groups:control group,CUS group,and 2groups of CUS that received different doses of MC extract(40 or 80 mg/kg).In treated groups,rats were given MC daily,followed by induction of CUS(1 stressor was randomly applied from a battery of 9 potential stressors)for 60 consecutive days.Plasma corticosterone and testosterone levels were analyzed after the end of experiment.Expressions of heat-shock protein 70(HSP-70)and tyrosine-phosphorylated proteins present in the fluid of the head and tail of the epididymis were quantified using Western blot.Results:MC extract contained TPC of(19.005±0.270)mg gallic acid equivalents and TFC of(0.306±0.012)mg catechin equivalents per gram,and had 2,2-diphenyl-1-picrylhydrazyl antioxidant capacity of(4.985±0.086)mg trolox equivalents per gram,radical 50%inhibitory concentration of(2.011±0.008)mg/mL and ferric reducing antioxidant power of(23.697±0.819)μmol Fe(Ⅱ)per gram.Testosterone level in the epididymis was significantly increased,while the corticosterone level was significantly improved in groups treated with MC extract,compared to the CUS animals.Particularly,an 80 mg/kg dose of MC extract prevented the impairments of HSP-70 and tyrosine-phosphorylated protein expressions in the luminal fluid of the epididymis of CUS rats.Conclusion:MC fruit extract had antioxidant activities and improved the functional proteins secreted from the head and tail of the epididymis.It i

关 键 词：Momordica charantia L. Antioxidant activity EPIDIDYMIS Tyrosine-phosphorylated proteins Chronic unpredictable stress 

分 类 号：R285.5[医药卫生—中药学]