pH急性胁迫对方斑东风螺行为及免疫酶活性的影响  

Effects of pH acute stress on the behavior and immune enzyme activity of Babylonia Areolata

作　　者：丁瑞霞 黄星美 赵旺 邓正华 陈恒大[3] 温为庚 马振华 王江勇[5] 郑忠明 DING Ruixia;HUANG Xingmei;ZHAO Wang;DENG Zhenghua;CHEN Hengda;WEN Weigeng;MA Zhenhua;WANG Jiangyong;ZHENG Zhongming(Tianjin Agricultural College,College of Fisheries,Tianjin 300384,China;Sanya Tropical Fisheries Research Institute,Sanya 572426,Hainan,China;Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province,Sanya 572426,Haian,China;Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization by the Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,Guangdong,China;Huizhou College,College of Life Sciences,Huizhou 516007,Guangdong,China;College of Oceanography,Ningbo University,Ningbo 315832,Zhejiang,China)

机构地区：[1]天津农学院水产学院,天津300384 [2]三亚热带水产研究院,海南三亚572426 [3]海南省深远海渔业资源高效利用与加工重点实验室,海南三亚,572426 [4]中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [5]惠州学院生命科学学院,广东惠州516007 [6]宁波大学海洋学院,浙江宁波315822

出　　处：《渔业现代化》2022年第6期84-90,共7页Fishery Modernization

基　　金：国家重点研发计划项目(2020YFD0900201);三亚市院地科技合作项目(2018YD19,2019YD21);现代农业产业技术体系专项资金(CARS-49);农业农村部财政专项(NHYYSWZZZYKZX2020);农业行业标准制定和修订项目(20191818);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2022YJ10;2020TD55);国家(省)重点科技项目三亚市配套资金(2019PT61);揭阳市科技计划重点项目(SKJCX020)。

摘　　要：为揭示方斑东风螺(Babylonia areolata)在低pH和高pH胁迫时的急性毒性和生理变化,以pH=8.0为对照组,pH=5.0、6.0、7.0和9.0为试验组,测定方斑东风螺体内4种免疫酶:谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)、过氧化物酶(POD)和酸性磷酸酶(ACP)活性。结果显示:不同时间pH胁迫对免疫酶活性有显著影响(P<0.05),而不同pH胁迫对免疫酶的活力没有显著影响(P>0.05);方斑东风螺经过pH急性胁迫后,在低pH和高pH的水体养殖6 h后都出现了活力下降、爬壁缓慢,而在48 h后开始出现死亡;各试验组GSH-PX活性均呈现“抑制-诱导”的变化趋势;随着时间的不断延长,各pH处理组CAT活力均呈现“诱导-抑制-诱导”的变化趋势;POD活性总体表现出了“诱导-抑制”的趋势;低pH处理组(pH=5.0、6.0)ACP活性表现出“诱导-抑制”的趋势。该研究对于方斑东风螺的养殖具有参考意义,同时丰富贝类免疫系统方面的基础资料。To reveal the acute toxicity and physiological changes of Babylonia areolata in response to low pH and high pH stress,the pH=8.0 control group and pH=5.0,6.0,7.0,and 9.0 were used as the experimental group to investigate the activities of four immune enzymes glutathione peroxidase(GSH-PX),catalase(CAT),peroxidase(POD)and acid phosphatase(ACP)in the body of the Babylonia areolata.The results of the experiment show that pH stress has a significant effect on immune enzyme activity at different times(P<0.05),while different pH stresses have no significant effect on immune enzyme activity(P>0.05).After pH acute stress,1)after 6h of low pH and high pH water culture,there is a decrease in vitality and slow climbing,and death begins to appear after 48h;2)The activity of GSH-PX in each experimental group shows a trend of"inhibition-induction";3)with the continuous extension of time,the CAT activity of each pH treatment group shows a trend of"induction-inhibition-induction";4)the POD activity generally show a trend of"induction-inhibition";5)the ACP activity of the low pH treatment group(pH=5.0,6.0)show a trend of"induction-inhibition".This study has practical significance for the cultivation of Babylonia areolata,and also enriches the basic data on the immune performance of other shellfish.

关 键 词：方斑东风螺(Babylonia areolata) 免疫酶 行为 pH急性胁迫 

分 类 号：S967.6[农业科学—水产养殖]