单细胞RNA测序应用于肾小球疾病研究的进展  

Advances in single-cell RNA sequencing in glomerular diseases

作　　者：邢海帆 范瑛[1] XING Haifan;FAN Ying()(Department of Nephrology,Shanghai Sixth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200233,China)

机构地区：[1]上海交通大学医学院附属第六人民医院肾内科,上海200233

出　　处：《上海交通大学学报（医学版）》2022年第10期1458-1465,共8页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81870468,82170727);上海交通大学转化医学国家重大科技基础设施(上海)开放课题基金(TMSK-2021-109)。

摘　　要：肾小球疾病是导致慢性肾脏病和终末期肾功能衰竭的主要原因。了解肾小球疾病发生和发展的分子机制对于早期识别疾病危险因素、挽救肾功能具有重要意义。肾脏中复杂且高度分化的细胞组成是肾小球疾病研究面临的一大挑战,传统测序方法无法确定特定细胞的转录变化以及不同类型细胞间的相互作用。近年来,单细胞RNA测序(single-cell RNA sequencing, scRNA-seq)技术发展迅速,可用于研究肾脏、尿液以及血液等不同来源细胞群体中单个细胞的基因表达模式。将scRNA-seq应用于肾小球疾病有助于生成全面的肾脏细胞图谱,从细胞层面解析肾小球疾病的复杂机制,开发预警生物标志物和细胞特异性的治疗方法。该文就scRNA-seq技术在临床常见原发性和继发性肾小球疾病中的应用研究进展进行综述。Glomerular diseases are a leading cause of chronic kidney disease and end-stage renal failure.Understanding the molecular mechanism underlying the initiation and progression of glomerular diseases is important to early identify risk factors for disease development and rescue renal function.The kidney is composed of complex and highly differentiated cells,which is a major challenge in the study of glomerular diseases.Bulk RNA sequencing cannot assess differential transcription or the interactions between cell types.Single-cell RNA sequencing(scRNA-seq) technology has developed rapidly in recent years,making it possible to study a wide range of cell types in kidney,urine and blood.The application of scRNA-seq to glomerular diseases helps to construct a comprehensive cell atlas,elucidate complex cellular and molecular mechanisms,develop efficient and accurate prognostic biomarkers and cell-specific therapies.This review focuses on the latest research advances in scRNA-seq in primary and secondary glomerular diseases.

关 键 词：单细胞RNA测序 转录组 肾小球肾炎 糖尿病肾病 狼疮性肾炎 IgA肾病 局灶节段性肾小球硬化 膜性肾病 

分 类 号：R692.6[医药卫生—泌尿科学]