小麦主要品质相关基因的分子检测与分析  

Molecular detection and analysis of main quality related genes in wheat

作　　者：孙华卫 凌冬[1] 张道荣[1] 唐清[1] 周芳菊[1] 汤清益[1] 刘先斌[1] 王志顺[1] 汤三明[1] 任喜峰 SUN Hua-wei;LING Dong;ZHANG Dao-rong;TANG Qing;ZHOU Fang-ju;TANG Qing-yi;LIU Xian-bin;WANG Zhi-shun;TANG San-ming;REN Xi-feng(Food Crop Research Institute,Xiangyang Academy of Agricultural Sciences,Xiangyang 441057,Hubei,China;Hubei Hongshan Laboratory,Wuhan 430070,China)

机构地区：[1]襄阳市农业科学院粮食作物研究所,湖北襄阳441057 [2]湖北洪山实验室,武汉430070

出　　处：《湖北农业科学》2022年第20期216-220,256,共6页Hubei Agricultural Sciences

基　　金：湖北省重点研发计划项目(2020BBA033);湖北洪山实验室开放课题(2021hskf009)。

摘　　要：为了明确相关品质基因在88份亲本材料中的分布和频率,为小麦品质育种提供亲本材料,利用分子标记对Ax2*、AxN、Bx7、By8、Dx5基因、多酚氧化酶基因以及1B/1R异位系进行检测。结果表明,高分子量谷蛋白亚基基因Ax2*、AxN、Bx7、By8、Dx5的分布频率分别为4.55%、57.95%、92.05%、35.23%和43.18%,1B/1R异位系频率为21.59%,多酚氧化酶基因Ppo-A1a(高PPO)、Ppo-A1b(低PPO)、Ppo-D1a(低PPO)和Ppo-D1b(高PPO)频率分别为45.45%、54.55%、55.68%和44.32%。基因型Ppo-A1b/Ppo-D1a(双低PPO)的频率为32.95%,基因型Ppo-A1a/Ppo-D1a(中等PPO)和Ppo-A1b/Ppo-D1b(中等PPO)的频率分别为22.73%和21.59%。基因型Ppo-A1a/Ppo-D1b(双高PPO)的频率为22.73%。聚合多个优质基因的亲本材料较少,同时含有7+8亚基、5+10亚基、非1B/1R易位以及双位点低PPO活性(Ppo-A1b/PpoD1a)的材料有3份,分别为郑麦119、郑麦578和藁优5766,可作为培育优质小麦的亲本材料。In order to clarify the distribution and frequency of related quality genes in 88 parental materials and provide parental materials for wheat quality breeding,molecular markers were used to detect the genes related to quality including Ax2*,AxN,Bx7,By8,Dx5 genes,polyphenol oxidase genes and 1B/1R translocation. The results showed that the distribution frequencies of Ax2*,AxN,Bx7,By8 and Dx5 genes were 4.55%,57.95%,92.05%,35.23% and 43.18%,respectively,and the frequency of 1B/1R translocation was 21.59%. The frequencies of polyphenol oxidase genes PPO-A1a(high PPO),PPO-A1b(low PPO),PPO-D1a(low PPO)and PPO-D1b(high PPO)were 45.45%,54.55%,55.68% and 44.32%,respectively. The frequency of genotype PPO-A1b/PPOD1a(double low PPO)was 32.95%,and the frequencies of genotype PPO-A1a/PPO-D1a(medium PPO)and PPO-A1b/PPO-D1b(medium PPO)were 22.73% and 21.59%,respectively. The frequency of genotype PPO-A1a/PPO-D1b(double high PPO)was22.73%. There were few parent materials polymering multiple high-quality genes. There were 3 materials containing 7+8,5+10 subunits,non-1B/1R translocation and double low PPO activity(ppo-A1b/ppo-D1a),which were Zhengmai 119,Zhengmai 578 and Gaoyou 5766,respectively,and could be used as parent materials for breeding high-quality wheat.

关 键 词：小麦 高分子量谷蛋白亚基 多酚氧化酶 1B/1R易位系 

分 类 号：S512[农业科学—作物学]