芦丁通过抑制NF-κB信号通路缓解大鼠结肠炎的研究  

Rutin relieves colitis in rats by inhibiting NF-κB signaling pathway

作　　者：郭建军[1] 凌应飞 李强 GUO Jianjun;LING Yingfei;LI Qiang(Department of Anorectal Medicine,Meishan Hospital of Traditional Chinese Medicine,Meishan,Sichuan 620000,China)

机构地区：[1]眉山市中医医院肛肠科,四川眉山620000

出　　处：《安徽医药》2023年第1期46-49,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨芦丁对右旋葡聚糖硫酸钠(DSS)诱导的大鼠溃疡性结肠炎(UC)的缓解作用以及对核因子κB(NF-κB)信号通路的影响。方法2020年4—5月建立右旋葡聚糖硫酸钠(DSS)诱导的UC模型。将大鼠分为对照组、模型组和芦丁组(20、40、80 mg/kg)。观察各组大鼠的疾病活动指数(DAI),比色法检测结肠组织髓过氧化物酶(MPO)活性,Elisa法检测大鼠结肠组织中的肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平,并采用蛋白质印迹法(western blotting)测定各组大鼠结肠组织的人磷酸化IκB激酶-α(p-IκBα)、IκB激酶-α(IκBα)以及核p65蛋白表达。结果芦丁高、中、低剂量组的DAI评分[(1.09±0.39)、(1.38±0.38)、(1.97±0.46)分]、MPO活性[(0.74±0.04)、(0.75±0.08)、(0.98±0.06)U/g]、TNF-α水平[(3.16±0.52)、(3.75±0.72)、(4.26±0.87)µg/L]和IL-6水平[(3.72±0.65)、(4.28±0.72)、(5.12±0.93)µg/L]均显著性低于模型组的(2.98±0.57)分、(1.26±0.07)U/g、(5.19±0.93)µg/L、(6.54±0.85)µg/L(P<0.05)。机制研究显示芦丁可以有效下调DSS诱导大鼠结肠组织的p-IκBα和核p65的水平的提高(P<0.05)。结论芦丁通过下调NF-κB信号通路缓解右旋葡聚糖硫酸钠诱导的大鼠溃疡性结肠炎。Objective To investigate the relief effect of rutin on dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)in rats and its effect on nuclear factor kappa-B(NF-κB)signaling pathway.Methods A rat model of Dextran Sulfate Sodium Salt(DSS)-in‐duced UC was established from April 2020 to May 2020.Rats were assigned into control group,model group and rutin group(20 mg/kg,40 mg/kg,80 mg/kg).The disease activity index(DAI)of rats in each group was observed,myeloperoxidase(MPO)activity of colon tis‐sue was detected using colorimetric method,tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in rat colon tissue were detected using Elisa method,and p-IκBα,IκBαand nuclear p65 protein in colon tissue were determined using Western blot.Results The DAI score[(1.09±0.39),(1.38±0.38),(1.97±0.46)],MPO activity[(0.74±0.04),(0.75±0.08),(0.98±0.06)U/g],TNF-αlevels[(3.16±0.52),(3.75±0.72),(4.26±0.87)µg/L]and IL-6 levels[(3.72±0.65),(4.28±0.72),(5.12±0.93)µg/L]of high,middle,low rutin group were all significantly lower than those of model group[(2.98±0.57),(1.26±0.07)U/g,(5.19±0.93)µg/L,(6.54±0.85)µg/L](P<0.05).The mecha‐nism study showed that rutin can effectively down-regulate DSS induced increase of p-IκBαand nuclear p65 in rat colon tissue(P<0.05).Conclusion Rutin alleviates dextran sodium sulfate-induced ulcerative colitis in rats by down-regulating the NF-κB signaling pathway.

关 键 词：芦丁 结肠炎 溃疡性 肿瘤坏死因子-Α 白细胞介素-6 核因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]